Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1618021..1618523 Replicon chromosome
Accession NZ_CP027352
Organism Escherichia coli strain 2012C-4606

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AYN42_RS09595 Protein ID WP_000767829.1
Coordinates 1618021..1618275 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AYN42_RS09600 Protein ID WP_001259255.1
Coordinates 1618272..1618523 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYN42_RS09565 1613036..1613263 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
AYN42_RS09570 1613277..1614335 - 1059 WP_000492324.1 transport protein YeeE -
AYN42_RS09575 1614514..1615872 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AYN42_RS09580 1615862..1615924 - 63 WP_010723108.1 membrane protein YoeI -
AYN42_RS09585 1616139..1617068 - 930 WP_000803351.1 LysR family transcriptional regulator -
AYN42_RS09590 1617114..1617938 - 825 WP_000754737.1 SDR family oxidoreductase -
AYN42_RS09595 1618021..1618275 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AYN42_RS09600 1618272..1618523 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AYN42_RS09605 1618806..1618853 + 48 WP_001375657.1 his operon leader peptide -
AYN42_RS09615 1618999..1619898 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
AYN42_RS09620 1619904..1621208 + 1305 WP_000009589.1 histidinol dehydrogenase -
AYN42_RS09625 1621205..1622275 + 1071 WP_000108914.1 histidinol-phosphate transaminase -
AYN42_RS09630 1622275..1623342 + 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T98233 WP_000767829.1 NZ_CP027352:c1618275-1618021 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T98233 NZ_CP027352:c1618275-1618021 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT98233 WP_001259255.1 NZ_CP027352:c1618523-1618272 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT98233 NZ_CP027352:c1618523-1618272 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References