Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3779301..3779803 Replicon chromosome
Accession NZ_CP027351
Organism Escherichia coli strain 2014C-3655

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ND05_RS20695 Protein ID WP_000767829.1
Coordinates 3779301..3779555 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ND05_RS20700 Protein ID WP_001259255.1
Coordinates 3779552..3779803 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ND05_RS20665 3774316..3774543 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
ND05_RS20670 3774557..3775615 - 1059 WP_000492305.1 transport protein YeeE -
ND05_RS20675 3775794..3777152 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ND05_RS20680 3777142..3777204 - 63 WP_010723108.1 membrane protein YoeI -
ND05_RS20685 3777419..3778348 - 930 WP_000803351.1 LysR family transcriptional regulator -
ND05_RS20690 3778394..3779218 - 825 WP_000754721.1 SDR family oxidoreductase -
ND05_RS20695 3779301..3779555 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ND05_RS20700 3779552..3779803 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ND05_RS20705 3780086..3780136 + 51 WP_001364200.1 his operon leader peptide -
ND05_RS20715 3780282..3781181 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ND05_RS20720 3781187..3782491 + 1305 WP_000009587.1 histidinol dehydrogenase -
ND05_RS20725 3782488..3783558 + 1071 WP_000108943.1 histidinol-phosphate transaminase -
ND05_RS20730 3783558..3784625 + 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T98209 WP_000767829.1 NZ_CP027351:c3779555-3779301 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T98209 NZ_CP027351:c3779555-3779301 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT98209 WP_001259255.1 NZ_CP027351:c3779803-3779552 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT98209 NZ_CP027351:c3779803-3779552 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References