Detailed information of TA system TA098196

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2915860..2916231
• Replicon: chromosome
• Accession: NZ_CP027351
• Organism: Escherichia coli strain 2014C-3655

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: ND05_RS15955
• Protein ID: WP_001317028.1
• Coordinates: 2916037..2916231 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2915860..2916038 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ND05_RS15925	2911612..2911785	+	174	WP_001296046.1	hypothetical protein	-
ND05_RS15930	2911815..2913188	+	1374	WP_000123758.1	ATP-dependent RNA helicase DbpA	-
ND05_RS15935	2913317..2914252	-	936	WP_001157421.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
ND05_RS15940	2914304..2915539	-	1236	WP_000040852.1	site-specific integrase	-
ND05_RS15945	2915541..2915756	-	216	WP_000079604.1	excisionase XisR	-
ND05_RS15950	2915835..2916044	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	2915860..2916038	+	179	NuclAT_0	-	Antitoxin
-	2915860..2916038	+	179	NuclAT_0	-	Antitoxin
-	2915860..2916038	+	179	NuclAT_0	-	Antitoxin
-	2915860..2916038	+	179	NuclAT_0	-	Antitoxin
ND05_RS15955	2916037..2916231	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
ND05_RS15960	2916288..2917097	-	810	WP_000595429.1	recombination protein RecT	-
ND05_RS15965	2917090..2919759	-	2670	WP_000102191.1	exodeoxyribonuclease VIII	-
ND05_RS15970	2919840..2920016	-	177	WP_000245534.1	hypothetical protein	-
ND05_RS15975	2920010..2920231	-	222	WP_000560223.1	killing protein KilR	-
ND05_RS15980	2920656..2920808	-	153	WP_001169149.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	2914304..2962543	48239

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T98196 WP_001317028.1 NZ_CP027351:c2916231-2916037 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T98196 NZ_CP027351:c2916231-2916037 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT98196 NZ_CP027351:2915860-2916038 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin