Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1541877..1542418 Replicon chromosome
Accession NZ_CP027351
Organism Escherichia coli strain 2014C-3655

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag ND05_RS08365 Protein ID WP_000615976.1
Coordinates 1541877..1542155 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A4T9JVH9
Locus tag ND05_RS08370 Protein ID WP_000729706.1
Coordinates 1542158..1542418 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ND05_RS08350 1539459..1540037 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
ND05_RS08355 1540243..1541010 + 768 WP_000333380.1 class II glutamine amidotransferase -
ND05_RS08360 1540981..1541721 - 741 WP_001225679.1 murein L,D-transpeptidase -
ND05_RS08365 1541877..1542155 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
ND05_RS08370 1542158..1542418 - 261 WP_000729706.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
ND05_RS08375 1542604..1543377 + 774 WP_000543899.1 C40 family peptidase -
ND05_RS08380 1543434..1543790 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
ND05_RS08385 1543783..1544061 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
ND05_RS08390 1544166..1545878 - 1713 Protein_1414 flagellar biosynthesis protein FlhA -
ND05_RS08395 1545850..1546635 + 786 WP_000207558.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T98191 WP_000615976.1 NZ_CP027351:c1542155-1541877 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T98191 NZ_CP027351:c1542155-1541877 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9390.79 Da        Isoelectric Point: 5.8129

>AT98191 WP_000729706.1 NZ_CP027351:c1542418-1542158 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADNL
FDKLGI

Download         Length: 261 bp

>AT98191 NZ_CP027351:c1542418-1542158 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATAATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
AlphaFold DB A0A4T9JVH9

References