Detailed information of TA system TA098183

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 22703..22972
• Replicon: plasmid unnamed
• Accession: NZ_CP027348
• Organism: Escherichia coli strain 2013C-4361

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AZF34_RS28425
• Protein ID: WP_001312861.1
• Coordinates: 22814..22972 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	22703..22768 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZF34_RS29100	17733..18023	+	291	WP_073972341.1	hypothetical protein	-
AZF34_RS28400	18496..19023	+	528	WP_000290792.1	single-stranded DNA-binding protein	-
AZF34_RS28405	19079..19312	+	234	WP_000006004.1	DUF905 domain-containing protein	-
AZF34_RS28410	19371..21329	+	1959	WP_001145472.1	ParB/RepB/Spo0J family partition protein	-
AZF34_RS28415	21384..21818	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
AZF34_RS28420	21815..22534	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
AZF34_RS29105	22546..22734	-	189	WP_001299721.1	hypothetical protein	-
-	22546..22770	+	225	NuclAT_0	-	-
-	22546..22770	+	225	NuclAT_0	-	-
-	22546..22770	+	225	NuclAT_0	-	-
-	22546..22770	+	225	NuclAT_0	-	-
-	22703..22768	+	66	-	-	Antitoxin
AZF34_RS28425	22814..22972	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
AZF34_RS29340	23326..23550	-	225	WP_001427866.1	hypothetical protein	-
AZF34_RS28445	23894..24181	+	288	WP_000107535.1	hypothetical protein	-
AZF34_RS28450	24301..25122	+	822	WP_001234469.1	DUF945 domain-containing protein	-
AZF34_RS28455	25419..25928	-	510	Protein_30	transglycosylase SLT domain-containing protein	-
AZF34_RS28460	26342..26724	+	383	Protein_31	relaxosome protein TraM	-
AZF34_RS28465	26911..27600	+	690	WP_000283394.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP / hlyD / hlyD / hlyB / hlyA / hlyC / hlyC	1..70613	70613

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T98183 WP_001312861.1 NZ_CP027348:22814-22972 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T98183 NZ_CP027348:22814-22972 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT98183 NZ_CP027348:22703-22768 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin