98181

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 5126118..5126659 Replicon chromosome
Accession NZ_CP027347
Organism Escherichia coli strain 2013C-4361

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag AZF34_RS27230 Protein ID WP_000615976.1
Coordinates 5126118..5126396 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AZF34_RS27235 Protein ID WP_000729704.1
Coordinates 5126399..5126659 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AZF34_RS27215 5123701..5124279 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
AZF34_RS27220 5124485..5125251 + 767 Protein_4970 class II glutamine amidotransferase -
AZF34_RS27225 5125222..5125962 - 741 WP_001225679.1 murein L,D-transpeptidase -
AZF34_RS27230 5126118..5126396 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AZF34_RS27235 5126399..5126659 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AZF34_RS27240 5126845..5127618 + 774 WP_000543898.1 C40 family peptidase -
AZF34_RS27245 5127675..5128031 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
AZF34_RS27250 5128024..5128302 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
AZF34_RS27255 5128407..5130500 - 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
AZF34_RS27260 5130484..5131623 - 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T98181 WP_000615976.1 NZ_CP027347:c5126396-5126118 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T98181 NZ_CP027347:c5126396-5126118 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT98181 WP_000729704.1 NZ_CP027347:c5126659-5126399 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT98181 NZ_CP027347:c5126659-5126399 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6Z6

References