Detailed information of TA system TA098177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4242634..4243236
• Replicon: chromosome
• Accession: NZ_CP027347
• Organism: Escherichia coli strain 2013C-4361

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AZF34_RS22805
• Protein ID: WP_000897305.1
• Coordinates: 4242634..4242945 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AZF34_RS22810
• Protein ID: WP_000356397.1
• Coordinates: 4242946..4243236 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZF34_RS22775	4237664..4238449	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
AZF34_RS22780	4238548..4239147	+	600	WP_001315111.1	glucose-1-phosphatase	-
AZF34_RS22785	4239141..4240013	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AZF34_RS22790	4240010..4240447	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AZF34_RS22795	4240492..4241433	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AZF34_RS22800	4241497..4242405	-	909	WP_001162704.1	alpha/beta hydrolase	-
AZF34_RS22805	4242634..4242945	+	312	WP_000897305.1	hypothetical protein	Toxin
AZF34_RS22810	4242946..4243236	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AZF34_RS22820	4243841..4244059	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
AZF34_RS22825	4244278..4244520	+	243	WP_001086388.1	hypothetical protein	-
AZF34_RS22830	4244851..4245780	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AZF34_RS22835	4245777..4246412	-	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
AZF34_RS22840	4246409..4247311	-	903	WP_000331369.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T98177 WP_000897305.1 NZ_CP027347:4242634-4242945 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T98177 NZ_CP027347:4242634-4242945 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT98177 WP_000356397.1 NZ_CP027347:4242946-4243236 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT98177 NZ_CP027347:4242946-4243236 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7