Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2069294..2069796 Replicon chromosome
Accession NZ_CP027347
Organism Escherichia coli strain 2013C-4361

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AZF34_RS11550 Protein ID WP_000767829.1
Coordinates 2069294..2069548 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AZF34_RS11555 Protein ID WP_001259255.1
Coordinates 2069545..2069796 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AZF34_RS11520 2064309..2064536 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
AZF34_RS11525 2064550..2065608 - 1059 WP_000492340.1 transport protein YeeE -
AZF34_RS11530 2065787..2067145 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AZF34_RS11535 2067135..2067197 - 63 WP_010723108.1 membrane protein YoeI -
AZF34_RS11540 2067412..2068341 - 930 WP_000803331.1 LysR family transcriptional regulator -
AZF34_RS11545 2068387..2069211 - 825 WP_000754755.1 SDR family oxidoreductase -
AZF34_RS11550 2069294..2069548 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AZF34_RS11555 2069545..2069796 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AZF34_RS11560 2070080..2070130 + 51 WP_001364200.1 his operon leader peptide -
AZF34_RS11570 2070276..2071175 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
AZF34_RS11575 2071181..2072485 + 1305 WP_001345258.1 histidinol dehydrogenase -
AZF34_RS11580 2072482..2073561 + 1080 WP_000102292.1 histidinol-phosphate transaminase -
AZF34_RS11585 2073558..2074625 + 1068 WP_000080054.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T98171 WP_000767829.1 NZ_CP027347:c2069548-2069294 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T98171 NZ_CP027347:c2069548-2069294 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT98171 WP_001259255.1 NZ_CP027347:c2069796-2069545 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT98171 NZ_CP027347:c2069796-2069545 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References