98171

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2069294..2069796 Replicon chromosome
Accession NZ_CP027347
Organism Escherichia coli strain 2013C-4361

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AZF34_RS11550 Protein ID WP_000767829.1
Coordinates 2069294..2069548 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AZF34_RS11555 Protein ID WP_001259255.1
Coordinates 2069545..2069796 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AZF34_RS11520 2064309..2064536 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
AZF34_RS11525 2064550..2065608 - 1059 WP_000492340.1 transport protein YeeE -
AZF34_RS11530 2065787..2067145 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AZF34_RS11535 2067135..2067197 - 63 WP_010723108.1 membrane protein YoeI -
AZF34_RS11540 2067412..2068341 - 930 WP_000803331.1 LysR family transcriptional regulator -
AZF34_RS11545 2068387..2069211 - 825 WP_000754755.1 SDR family oxidoreductase -
AZF34_RS11550 2069294..2069548 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AZF34_RS11555 2069545..2069796 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AZF34_RS11560 2070080..2070130 + 51 WP_001364200.1 his operon leader peptide -
AZF34_RS11570 2070276..2071175 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
AZF34_RS11575 2071181..2072485 + 1305 WP_001345258.1 histidinol dehydrogenase -
AZF34_RS11580 2072482..2073561 + 1080 WP_000102292.1 histidinol-phosphate transaminase -
AZF34_RS11585 2073558..2074625 + 1068 WP_000080054.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T98171 WP_000767829.1 NZ_CP027347:c2069548-2069294 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T98171 NZ_CP027347:c2069548-2069294 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT98171 WP_001259255.1 NZ_CP027347:c2069796-2069545 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT98171 NZ_CP027347:c2069796-2069545 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References