Detailed information of TA system TA098146

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 24259..24679
• Replicon: plasmid unnamed
• Accession: NZ_CP027343
• Organism: Escherichia coli strain 2014C-4587

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: C6N23_RS26305
• Protein ID: WP_000775238.1
• Coordinates: 24259..24420 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	24486..24679 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N23_RS26260	20065..20448	-	384	WP_001151560.1	relaxosome protein TraM	-
C6N23_RS26265	20733..21380	+	648	WP_135301269.1	transglycosylase SLT domain-containing protein	-
C6N23_RS26270	21676..22497	-	822	WP_052892104.1	DUF945 domain-containing protein	-
C6N23_RS26275	22616..22903	-	288	WP_106914345.1	hypothetical protein	-
C6N23_RS26285	23059..23361	-	303	WP_001272235.1	hypothetical protein	-
C6N23_RS27325	23661..24021	+	361	Protein_36	hypothetical protein	-
C6N23_RS26305	24259..24420	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	24486..24679	-	194	NuclAT_0	-	Antitoxin
-	24486..24679	-	194	NuclAT_0	-	Antitoxin
-	24486..24679	-	194	NuclAT_0	-	Antitoxin
-	24486..24679	-	194	NuclAT_0	-	Antitoxin
C6N23_RS26310	24628..24942	-	315	WP_000872086.1	hypothetical protein	-
C6N23_RS26315	24939..25658	-	720	WP_001276237.1	plasmid SOS inhibition protein A	-
C6N23_RS26320	25655..26089	-	435	WP_000845923.1	conjugation system SOS inhibitor PsiB	-
C6N23_RS26325	26144..28102	-	1959	WP_052892092.1	ParB/RepB/Spo0J family partition protein	-
C6N23_RS26330	28163..28396	-	234	WP_000005993.1	DUF905 family protein	-
C6N23_RS26340	28454..28975	-	522	WP_032107538.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / espP	1..131410	131410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T98146 WP_000775238.1 NZ_CP027343:c24420-24259 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T98146 NZ_CP027343:c24420-24259 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 194 bp

>AT98146 NZ_CP027343:c24679-24486 [Escherichia coli]
ACTGATTGCCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGTGCGCAGGTATATGGGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGCAGAA
AGCCCCGTAGTTAATTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin