Detailed information of TA system TA098126

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 572804..573406
• Replicon: chromosome
• Accession: NZ_CP027342
• Organism: Escherichia coli strain 2014C-4587

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6N23_RS02860
• Protein ID: WP_000897305.1
• Coordinates: 573095..573406 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6N23_RS02855
• Protein ID: WP_000356397.1
• Coordinates: 572804..573094 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N23_RS02825	568730..569632	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
C6N23_RS02830	569629..570264	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C6N23_RS02835	570261..571190	+	930	WP_000027707.1	formate dehydrogenase accessory protein FdhE	-
C6N23_RS02840	571520..571762	-	243	WP_001086388.1	hypothetical protein	-
C6N23_RS02845	571981..572199	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C6N23_RS02855	572804..573094	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6N23_RS02860	573095..573406	-	312	WP_000897305.1	hypothetical protein	Toxin
C6N23_RS02865	573635..574543	+	909	WP_001162704.1	alpha/beta hydrolase	-
C6N23_RS02870	574607..575548	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
C6N23_RS02875	575593..576030	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6N23_RS02880	576027..576899	-	873	WP_000920762.1	virulence factor BrkB family protein	-
C6N23_RS02885	576893..577492	-	600	WP_001295269.1	glucose-1-phosphatase	-
C6N23_RS02890	577591..578376	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T98126 WP_000897305.1 NZ_CP027342:c573406-573095 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T98126 NZ_CP027342:c573406-573095 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT98126 WP_000356397.1 NZ_CP027342:c573094-572804 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT98126 NZ_CP027342:c573094-572804 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7