Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4518383..4518885 Replicon chromosome
Accession NZ_CP027340
Organism Escherichia coli strain 2015C-3121

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C6N25_RS24670 Protein ID WP_000767829.1
Coordinates 4518383..4518637 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C6N25_RS24675 Protein ID WP_001259255.1
Coordinates 4518634..4518885 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N25_RS24640 4513443..4513625 - 183 WP_000985269.1 sulfurtransferase TusA family protein -
C6N25_RS24645 4513639..4514697 - 1059 WP_000492340.1 transport protein YeeE -
C6N25_RS24650 4514876..4516234 - 1359 Protein_4440 putrescine/proton symporter PlaP -
C6N25_RS24655 4516224..4516286 - 63 WP_010723108.1 membrane protein YoeI -
C6N25_RS24660 4516501..4517430 - 930 WP_000803351.1 LysR family transcriptional regulator -
C6N25_RS24665 4517476..4518300 - 825 WP_000754737.1 SDR family oxidoreductase -
C6N25_RS24670 4518383..4518637 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C6N25_RS24675 4518634..4518885 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C6N25_RS24680 4519168..4519218 + 51 WP_001364200.1 his operon leader peptide -
C6N25_RS24690 4519364..4520263 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C6N25_RS24695 4520269..4521573 + 1305 WP_001368033.1 histidinol dehydrogenase -
C6N25_RS24700 4521570..4522640 + 1071 WP_000108967.1 histidinol-phosphate transaminase -
C6N25_RS24705 4522640..4523707 + 1068 WP_000080131.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T98119 WP_000767829.1 NZ_CP027340:c4518637-4518383 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T98119 NZ_CP027340:c4518637-4518383 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT98119 WP_001259255.1 NZ_CP027340:c4518885-4518634 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT98119 NZ_CP027340:c4518885-4518634 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References