Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2471794..2472335 Replicon chromosome
Accession NZ_CP027340
Organism Escherichia coli strain 2015C-3121

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag C6N25_RS13215 Protein ID WP_000615976.1
Coordinates 2471794..2472072 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C6N25_RS13220 Protein ID WP_000729704.1
Coordinates 2472075..2472335 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N25_RS13200 2469376..2469954 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
C6N25_RS13205 2470160..2470927 + 768 WP_000333380.1 class II glutamine amidotransferase -
C6N25_RS13210 2470898..2471638 - 741 WP_001225685.1 murein L,D-transpeptidase -
C6N25_RS13215 2471794..2472072 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C6N25_RS13220 2472075..2472335 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C6N25_RS13225 2472521..2473294 + 774 WP_000543896.1 C40 family peptidase -
C6N25_RS13230 2473351..2473707 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
C6N25_RS13235 2473700..2473978 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
C6N25_RS13240 2474083..2475795 - 1713 Protein_2373 flagellar biosynthesis protein FlhA -
C6N25_RS13245 2475767..2476552 + 786 WP_000207589.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T98104 WP_000615976.1 NZ_CP027340:c2472072-2471794 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T98104 NZ_CP027340:c2472072-2471794 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT98104 WP_000729704.1 NZ_CP027340:c2472335-2472075 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT98104 NZ_CP027340:c2472335-2472075 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References