Detailed information of TA system TA098100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1374341..1374943
• Replicon: chromosome
• Accession: NZ_CP027340
• Organism: Escherichia coli strain 2015C-3121

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6N25_RS07390
• Protein ID: WP_000897305.1
• Coordinates: 1374341..1374652 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6N25_RS07395
• Protein ID: WP_000356397.1
• Coordinates: 1374653..1374943 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N25_RS07365	1370255..1370854	+	600	WP_001295269.1	glucose-1-phosphatase	-
C6N25_RS07370	1370848..1371720	+	873	WP_000920762.1	virulence factor BrkB family protein	-
C6N25_RS07375	1371717..1372154	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6N25_RS07380	1372199..1373140	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6N25_RS07385	1373204..1374112	-	909	WP_001162704.1	alpha/beta hydrolase	-
C6N25_RS07390	1374341..1374652	+	312	WP_000897305.1	hypothetical protein	Toxin
C6N25_RS07395	1374653..1374943	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6N25_RS07405	1375529..1375747	+	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
C6N25_RS07410	1375967..1376209	+	243	WP_001087409.1	hypothetical protein	-
C6N25_RS07415	1376539..1377468	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C6N25_RS07420	1377465..1378100	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C6N25_RS07425	1378097..1378999	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T98100 WP_000897305.1 NZ_CP027340:1374341-1374652 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T98100 NZ_CP027340:1374341-1374652 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT98100 WP_000356397.1 NZ_CP027340:1374653-1374943 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT98100 NZ_CP027340:1374653-1374943 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7