Detailed information of TA system TA098059

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 83953..84379
• Replicon: plasmid unnamed3
• Accession: NZ_CP027334
• Organism: Escherichia coli strain 2013C-3277

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: MJ63_RS30090
• Protein ID: WP_001312861.1
• Coordinates: 83953..84111 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	84155..84379 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ63_RS30060	78964..79311	+	348	WP_000624618.1	IS66 family insertion sequence element accessory protein TnpB	-
MJ63_RS30065	79331..80902	+	1572	WP_000381416.1	IS66 family transposase	-
MJ63_RS30070	80931..81200	+	270	Protein_94	protein RepA	-
MJ63_RS30075	81350..82889	-	1540	Protein_95	IS66-like element ISEc8 family transposase	-
MJ63_RS30080	82939..83285	-	347	Protein_96	IS66 family insertion sequence element accessory protein TnpB	-
MJ63_RS30085	83282..83662	-	381	WP_001171554.1	IS66 family insertion sequence hypothetical protein	-
MJ63_RS30090	83953..84111	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	84155..84379	-	225	NuclAT_0	-	Antitoxin
-	84155..84379	-	225	NuclAT_0	-	Antitoxin
-	84155..84379	-	225	NuclAT_0	-	Antitoxin
-	84155..84379	-	225	NuclAT_0	-	Antitoxin
MJ63_RS31115	84191..84379	+	189	WP_001336239.1	hypothetical protein	-
MJ63_RS30095	84391..85110	-	720	WP_032345718.1	plasmid SOS inhibition protein A	-
MJ63_RS30100	85107..85541	-	435	WP_032345719.1	conjugation system SOS inhibitor PsiB	-
MJ63_RS30105	85596..87415	-	1820	Protein_102	ParB/RepB/Spo0J family partition protein	-
MJ63_RS30110	87354..87680	+	327	WP_032345720.1	hypothetical protein	-
MJ63_RS30115	87780..87980	+	201	Protein_104	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / hlyA / hlyB / hlyD / hlyD / toxB / toxB / toxB / espP	1..181066	181066
	-	inside	IScluster/Tn	-	-	78314..90440	12126

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T98059 WP_001312861.1 NZ_CP027334:c84111-83953 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T98059 NZ_CP027334:c84111-83953 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT98059 NZ_CP027334:c84379-84155 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGTCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin