Detailed information of TA system TA098052

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 5690..5954
• Replicon: plasmid unnamed2
• Accession: NZ_CP027333
• Organism: Escherichia coli strain 2013C-3277

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: MJ63_RS29290
• Protein ID: WP_001331364.1
• Coordinates: 5802..5954 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	5690..5752 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ63_RS29270	926..1421	-	496	Protein_1	type IV secretion protein IcmO	-
MJ63_RS29280	3212..4282	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
MJ63_RS29285	4301..5510	-	1210	Protein_3	IncI1-type conjugal transfer protein TrbA	-
-	5690..5752	-	63	NuclAT_0	-	Antitoxin
-	5690..5752	-	63	NuclAT_0	-	Antitoxin
-	5690..5752	-	63	NuclAT_0	-	Antitoxin
-	5690..5752	-	63	NuclAT_0	-	Antitoxin
MJ63_RS29290	5802..5954	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
MJ63_RS29295	6026..6277	-	252	WP_001291964.1	hypothetical protein	-
MJ63_RS31345	6936..7112	-	177	WP_001054897.1	hypothetical protein	-
MJ63_RS29300	7504..7713	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
MJ63_RS31090	7785..8436	-	652	Protein_8	plasmid IncI1-type surface exclusion protein ExcA	-
MJ63_RS29315	8510..10678	-	2169	WP_000698357.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..46866	46866

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T98052 WP_001331364.1 NZ_CP027333:5802-5954 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T98052 NZ_CP027333:5802-5954 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT98052 NZ_CP027333:c5752-5690 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin