Detailed information of TA system TA098026

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralR-sib/-
• Location: 1576236..1576607
• Replicon: chromosome
• Accession: NZ_CP027331
• Organism: Escherichia coli strain 2013C-3277

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: -
• Locus tag: MJ63_RS08930
• Protein ID: WP_050439817.1
• Coordinates: 1576236..1576430 (+)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	sib
Locus tag	-
Coordinates	1576429..1576607 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ63_RS08900	1571314..1571466	+	153	WP_001427316.1	DUF1391 family protein	-
MJ63_RS08905	1571887..1572108	+	222	WP_000560218.1	killing protein KilR	-
MJ63_RS08910	1572102..1572278	+	177	WP_000245534.1	hypothetical protein	-
MJ63_RS08915	1572352..1572627	+	276	WP_001307773.1	hypothetical protein	-
MJ63_RS08920	1572726..1575377	+	2652	WP_032346644.1	exodeoxyribonuclease VIII	-
MJ63_RS08925	1575370..1576179	+	810	WP_000166317.1	recombination protein RecT	-
MJ63_RS08930	1576236..1576430	+	195	WP_050439817.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
MJ63_RS08935	1576423..1576611	+	189	WP_001356607.1	DUF1187 family protein	-
-	1576429..1576607	-	179	NuclAT_1	-	Antitoxin
-	1576429..1576607	-	179	NuclAT_1	-	Antitoxin
-	1576429..1576607	-	179	NuclAT_1	-	Antitoxin
-	1576429..1576607	-	179	NuclAT_1	-	Antitoxin
MJ63_RS08940	1576718..1576999	+	282	WP_001443927.1	excisionase	-
MJ63_RS08945	1576965..1578041	+	1077	WP_001189091.1	phage integrase Arm DNA-binding domain-containing protein	-
MJ63_RS08950	1578434..1578775	-	342	WP_000976492.1	YebY family protein	-
MJ63_RS08955	1578788..1579660	-	873	WP_000879280.1	copper homeostasis membrane protein CopD	-
MJ63_RS08960	1579664..1580038	-	375	WP_000204699.1	CopC domain-containing protein YobA	-
MJ63_RS08965	1580177..1580407	+	231	WP_000916763.1	DNA polymerase III subunit theta	-
MJ63_RS08970	1580509..1581165	+	657	WP_000011658.1	carbon-nitrogen hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	espFu/tccP	1531673..1583908	52235

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7077.91 Da
• Isoelectric Point: 8.3431

>T98026 WP_050439817.1 NZ_CP027331:1576236-1576430 [Escherichia coli]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEELERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T98026 NZ_CP027331:1576236-1576430 [Escherichia coli]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCTGTTACAGTGAAAGCAATTTCAGGATATTACCG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGAACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT98026 NZ_CP027331:c1576607-1576429 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin