Detailed information of TA system TA098019

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralR-sib/-
• Location: 964219..964590
• Replicon: chromosome
• Accession: NZ_CP027331
• Organism: Escherichia coli strain 2013C-3277

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: MJ63_RS05545
• Protein ID: WP_021500490.1
• Coordinates: 964396..964590 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	sib
Locus tag	-
Coordinates	964219..964397 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ63_RS05520	960174..961547	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
MJ63_RS05525	961676..962611	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
MJ63_RS05530	962664..963898	-	1235	Protein_1010	site-specific integrase	-
MJ63_RS05535	963900..964115	-	216	WP_000079604.1	excisionase XisR	-
MJ63_RS05540	964215..964403	-	189	WP_001298826.1	DUF1187 family protein	-
-	964219..964397	+	179	NuclAT_0	-	Antitoxin
-	964219..964397	+	179	NuclAT_0	-	Antitoxin
-	964219..964397	+	179	NuclAT_0	-	Antitoxin
-	964219..964397	+	179	NuclAT_0	-	Antitoxin
MJ63_RS05545	964396..964590	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
MJ63_RS05550	964654..965706	-	1053	WP_001004423.1	recombinase RecT	-
MJ63_RS05555	965718..968845	-	3128	Protein_1015	exodeoxyribonuclease VIII	-
MJ63_RS05560	968947..969222	-	276	WP_001519830.1	phage protein	-
MJ63_RS05565	969297..969473	-	177	WP_000245528.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	955404..1030567	75163

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T98019 WP_021500490.1 NZ_CP027331:c964590-964396 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T98019 NZ_CP027331:c964590-964396 [Escherichia coli]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT98019 NZ_CP027331:964219-964397 [Escherichia coli]
GAGGACTGAAGTTGCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin