Detailed information of TA system TA098012

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69796..70060
• Replicon: plasmid unnamed2
• Accession: NZ_CP027327
• Organism: Escherichia coli strain 2013C-4830

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: C6N65_RS28245
• Protein ID: WP_001303307.1
• Coordinates: 69796..69948 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	70003..70060 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N65_RS28225	65264..67432	+	2169	WP_063267537.1	IncI1-type conjugal transfer membrane protein TraY	-
C6N65_RS28230	67508..68122	+	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
C6N65_RS28235	68220..68429	+	210	WP_001140545.1	hemolysin expression modulator Hha	-
C6N65_RS28990	68638..68814	+	177	WP_001054897.1	hypothetical protein	-
C6N65_RS28240	69473..69724	+	252	WP_001291965.1	hypothetical protein	-
C6N65_RS28245	69796..69948	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
-	70003..70060	+	58	NuclAT_0	-	Antitoxin
-	70003..70060	+	58	NuclAT_0	-	Antitoxin
-	70003..70060	+	58	NuclAT_0	-	Antitoxin
-	70003..70060	+	58	NuclAT_0	-	Antitoxin
C6N65_RS28250	70240..71448	+	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
C6N65_RS28255	71467..72537	+	1071	WP_059277929.1	IncI1-type conjugal transfer protein TrbB	-
C6N65_RS28995	72530..73675	+	1146	WP_199852764.1	hypothetical protein	-
C6N65_RS29000	73996..74820	+	825	WP_199852765.1	icmO-like type IV secretion system domain protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..93170	93170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T98012 WP_001303307.1 NZ_CP027327:c69948-69796 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T98012 NZ_CP027327:c69948-69796 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT98012 NZ_CP027327:70003-70060 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin