Detailed information of TA system TA098006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5050859..5051586
• Replicon: chromosome
• Accession: NZ_CP027325
• Organism: Escherichia coli strain 2013C-4830

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: C6N65_RS26935
• Protein ID: WP_000547563.1
• Coordinates: 5051275..5051586 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6N65_RS26930
• Protein ID: WP_000126296.1
• Coordinates: 5050859..5051278 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N65_RS26910	5045908..5046435	-	528	WP_032207572.1	electron transport protein HydN	-
C6N65_RS26915	5046584..5047594	-	1011	WP_001374632.1	DNA-binding transcriptional regulator AscG	-
C6N65_RS26920	5047854..5049311	+	1458	WP_032207573.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
C6N65_RS26925	5049320..5050744	+	1425	WP_032207736.1	6-phospho-beta-glucosidase AscB	-
C6N65_RS26930	5050859..5051278	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
C6N65_RS26935	5051275..5051586	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
C6N65_RS26940	5051748..5052503	-	756	WP_032207575.1	hypothetical protein	-
C6N65_RS26945	5052546..5053016	-	471	WP_032207577.1	hydrogenase maturation peptidase HycI	-
C6N65_RS26950	5053009..5053419	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
C6N65_RS26955	5053416..5054183	-	768	WP_032207579.1	formate hydrogenlyase subunit HycG	-
C6N65_RS26960	5054183..5054725	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
C6N65_RS26965	5054735..5056444	-	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T98006 WP_000547563.1 NZ_CP027325:c5051586-5051275 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T98006 NZ_CP027325:c5051586-5051275 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAATAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT98006 WP_000126296.1 NZ_CP027325:c5051278-5050859 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT98006 NZ_CP027325:c5051278-5050859 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22