Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4322862..4323364 Replicon chromosome
Accession NZ_CP027325
Organism Escherichia coli strain 2013C-4830

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C6N65_RS23330 Protein ID WP_000767829.1
Coordinates 4322862..4323116 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C6N65_RS23335 Protein ID WP_001259255.1
Coordinates 4323113..4323364 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N65_RS23300 4317877..4318104 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
C6N65_RS23305 4318118..4319176 - 1059 WP_032206762.1 transport protein YeeE -
C6N65_RS23310 4319355..4320713 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C6N65_RS23315 4320703..4320765 - 63 WP_010723108.1 membrane protein YoeI -
C6N65_RS23320 4320980..4321909 - 930 WP_032206761.1 LysR family transcriptional regulator -
C6N65_RS23325 4321955..4322779 - 825 WP_000754749.1 SDR family oxidoreductase -
C6N65_RS23330 4322862..4323116 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C6N65_RS23335 4323113..4323364 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C6N65_RS23340 4323647..4323697 + 51 WP_001364200.1 his operon leader peptide -
C6N65_RS23350 4323843..4324742 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C6N65_RS23355 4324748..4326052 + 1305 WP_032206759.1 histidinol dehydrogenase -
C6N65_RS23360 4326049..4327119 + 1071 WP_000108943.1 histidinol-phosphate transaminase -
C6N65_RS23365 4327119..4328186 + 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T98004 WP_000767829.1 NZ_CP027325:c4323116-4322862 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T98004 NZ_CP027325:c4323116-4322862 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT98004 WP_001259255.1 NZ_CP027325:c4323364-4323113 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT98004 NZ_CP027325:c4323364-4323113 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References