98004

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4322862..4323364 Replicon chromosome
Accession NZ_CP027325
Organism Escherichia coli strain 2013C-4830

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C6N65_RS23330 Protein ID WP_000767829.1
Coordinates 4322862..4323116 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C6N65_RS23335 Protein ID WP_001259255.1
Coordinates 4323113..4323364 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N65_RS23300 4317877..4318104 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
C6N65_RS23305 4318118..4319176 - 1059 WP_032206762.1 transport protein YeeE -
C6N65_RS23310 4319355..4320713 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C6N65_RS23315 4320703..4320765 - 63 WP_010723108.1 membrane protein YoeI -
C6N65_RS23320 4320980..4321909 - 930 WP_032206761.1 LysR family transcriptional regulator -
C6N65_RS23325 4321955..4322779 - 825 WP_000754749.1 SDR family oxidoreductase -
C6N65_RS23330 4322862..4323116 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C6N65_RS23335 4323113..4323364 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C6N65_RS23340 4323647..4323697 + 51 WP_001364200.1 his operon leader peptide -
C6N65_RS23350 4323843..4324742 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C6N65_RS23355 4324748..4326052 + 1305 WP_032206759.1 histidinol dehydrogenase -
C6N65_RS23360 4326049..4327119 + 1071 WP_000108943.1 histidinol-phosphate transaminase -
C6N65_RS23365 4327119..4328186 + 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T98004 WP_000767829.1 NZ_CP027325:c4323116-4322862 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T98004 NZ_CP027325:c4323116-4322862 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT98004 WP_001259255.1 NZ_CP027325:c4323364-4323113 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT98004 NZ_CP027325:c4323364-4323113 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References