Detailed information of TA system TA000098

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/RHH-PIN
• Location: 1947030..1947643
• Replicon: chromosome
• Accession: NC_000962
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFA3
• Locus tag: Rv1720c
• Protein ID: 15608858
• Coordinates: 1947030..1947419 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ53
• Locus tag: Rv1721c
• Protein ID: 57116902
• Coordinates: 1947416..1947643 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Rv1715	1942659..1943573	+	915	YP_177829.1	3-hydroxybutyryl-CoA dehydrogenase FadB	-
Rv1716	1943576..1944406	+	831	NP_216232.1	hypothetical protein	-
Rv1717	1944406..1944756	+	351	NP_216233.1	hypothetical protein	-
Rv1718	1944809..1945627	+	819	NP_216234.1	hypothetical protein	-
Rv1719	1945641..1946420	+	780	NP_216235.1	transcriptional regulator	-
Rv1720c	1947030..1947419	-	390	NP_216236.1	ribonuclease VapC12	Toxin
Rv1721c	1947416..1947643	-	228	NP_216237.2	antitoxin VapB12	Antitoxin
Rv1722	1947861..1949345	+	1485	NP_216238.1	carboxylase	-
Rv1723	1949342..1950589	+	1248	NP_216239.1	hydrolase	-
Rv1724c	1950632..1951051	-	420	NP_216240.1	hypothetical protein	-
Rv1725c	1951041..1951751	-	711	NP_216241.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-116)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 13817.98 Da
• Isoelectric Point: 7.0612

>T98 15608858 NC_000962:c1947419-1947030 [Mycobacterium tuberculosis H37Rv]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Nucleotide

• Length: 390 bp

>T98 NC_000962:c1947419-1947030 [Mycobacterium tuberculosis H37Rv]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8226.39 Da
• Isoelectric Point: 4.3313

>AT98 57116902 NC_000962:c1947643-1947416 [Mycobacterium tuberculosis H37Rv]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Nucleotide

• Length: 228 bp

>AT98 NC_000962:c1947643-1947416 [Mycobacterium tuberculosis H37Rv]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T6241	Mycobacterium tuberculosis H37Rv	39.062	99.225	0.388
T75	Mycobacterium tuberculosis H37Rv	35.593	91.473	0.326

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: P9WFA3

Antitoxin

• AlphaFold DB: P9WJ53

References

(1) Sakshi Talwar et al. (2020) Role of VapBC12 Toxin-Antitoxin Locus in Cholesterol-Induced Mycobacterial Persistence. MSystems 5(6):e00855-20. [PubMed:33323416]


 experimental literature