Detailed information of TA system TA006241

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/RHH-PIN
• Location: 71589..72222
• Replicon: chromosome
• Accession: NC_000962
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFC1
• Locus tag: Rv0065
• Protein ID: NP_214579.1
• Coordinates: 71821..72222 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P0CW29
• Locus tag: Rv0064A
• Protein ID: YP_007408701.1
• Coordinates: 71589..71828 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Rv0063	66923..68362	+	1440	NP_214577.1	oxidoreductase	-
Rv0063a	68418..68579	+	162	YP_009030026.1	hypothetical protein	-
Rv0064	68620..71559	+	2940	NP_214578.1	transmembrane protein	-
Rv0064A	71589..71828	+	240	YP_007408701.1	antitoxin VapB1	Antitoxin
Rv0065	71821..72222	+	402	NP_214579.1	ribonuclease VapC1	Toxin
Rv0066c	72274..74511	-	2238	NP_214580.1	isocitrate dehydrogenase	-
Rv0067c	74629..75198	-	570	NP_214581.1	transcriptional regulator	-
Rv0068	75301..76212	+	912	NP_214582.1	oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (36-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14339.44 Da
• Isoelectric Point: 4.8887

>T6241 NP_214579.1 NC_000962:71821-72222 [Mycobacterium tuberculosis H37Rv]
VDECVVDAAAVVDALAGKGASAIVLRGLLKESISNAPHLLDAEVGHALRRAVLSDEISEEQARAALDALPYLIDNRYPHS
PRLIEYTWQLRHNVTFYDALYVALATALDVPLLTGDSRLAAAPGLPCEIKLVR

Nucleotide

• Length: 402 bp

>T6241 NC_000962:71821-72222 [Mycobacterium tuberculosis H37Rv]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8717.84 Da
• Isoelectric Point: 8.5960

>AT6241 YP_007408701.1 NC_000962:71589-71828 [Mycobacterium tuberculosis H37Rv]
MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEAIEILEQALASTASPGISRETIEASRRELRGG

Nucleotide

• Length: 240 bp

>AT6241 NC_000962:71589-71828 [Mycobacterium tuberculosis H37Rv]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGCAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T98	Mycobacterium tuberculosis H37Rv	39.062	99.225	0.388
T75	Mycobacterium tuberculosis H37Rv	36.364	88.321	0.321

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: P9WFC1

Antitoxin

• AlphaFold DB: P0CW29

References

(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]

(2) Abigail Sharrock et al. (2018) VapC proteins from Mycobacterium tuberculosis share ribonuclease sequence specificity but differ in regulation and toxicity. PloS One 13(8):e0203412. [PubMed:30169502]


 experimental literature