97990

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2138742..2139283 Replicon chromosome
Accession NZ_CP027325
Organism Escherichia coli strain 2013C-4830

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag C6N65_RS11135 Protein ID WP_000615979.1
Coordinates 2138742..2139020 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C6N65_RS11140 Protein ID WP_000729704.1
Coordinates 2139023..2139283 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N65_RS11120 2136324..2136902 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
C6N65_RS11125 2137108..2137875 + 768 WP_001365365.1 class II glutamine amidotransferase -
C6N65_RS11130 2137846..2138586 - 741 WP_001225679.1 murein L,D-transpeptidase -
C6N65_RS11135 2138742..2139020 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C6N65_RS11140 2139023..2139283 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C6N65_RS11145 2139469..2140242 + 774 WP_000543899.1 C40 family peptidase -
C6N65_RS11150 2140299..2140655 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
C6N65_RS11155 2140648..2140926 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
C6N65_RS11160 2141031..2142743 - 1713 Protein_2014 flagellar biosynthesis protein FlhA -
C6N65_RS11165 2142715..2143500 + 786 WP_062875323.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - hcp1/tssD1 / tssA / rhs/PAAR / gmhA/lpcA 2115022..2147767 32745


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T97990 WP_000615979.1 NZ_CP027325:c2139020-2138742 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T97990 NZ_CP027325:c2139020-2138742 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT97990 WP_000729704.1 NZ_CP027325:c2139283-2139023 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT97990 NZ_CP027325:c2139283-2139023 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6Z6

References