Detailed information of TA system TA097985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 419804..420603
• Replicon: chromosome
• Accession: NZ_CP027325
• Organism: Escherichia coli strain 2013C-4830

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: C6N65_RS02185
• Protein ID: WP_032208138.1
• Coordinates: 420139..420603 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: C6N65_RS02180
• Protein ID: WP_032208136.1
• Coordinates: 419804..420139 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N65_RS02165	415589..416359	-	771	WP_032208131.1	2-dehydro-3-deoxyglucarate aldolase	-
C6N65_RS02170	416375..417709	-	1335	WP_032208133.1	galactarate/glucarate/glycerate transporter GarP	-
C6N65_RS02175	418084..419655	+	1572	WP_032208135.1	galactarate dehydratase	-
C6N65_RS02180	419804..420139	+	336	WP_032208136.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
C6N65_RS02185	420139..420603	+	465	WP_032208138.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
C6N65_RS02190	420658..421467	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
C6N65_RS02200	421716..422996	+	1281	WP_000681922.1	tagatose-bisphosphate aldolase subunit KbaZ	-
C6N65_RS02205	423019..423492	+	474	WP_032208140.1	PTS N-acetylgalactosamine transporter subunit IIB	-
C6N65_RS02210	423503..424282	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
C6N65_RS02215	424272..425150	+	879	WP_001341890.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
C6N65_RS02220	425168..425602	+	435	WP_032208141.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	409160..420603	11443

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17837.19 Da
• Isoelectric Point: 9.4947

>T97985 WP_032208138.1 NZ_CP027325:420139-420603 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGASRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T97985 NZ_CP027325:420139-420603 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTAGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12405.01 Da
• Isoelectric Point: 4.8616

>AT97985 WP_032208136.1 NZ_CP027325:419804-420139 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKLGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT97985 NZ_CP027325:419804-420139 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCTAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA