Detailed information of TA system TA097980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4191..4837
• Replicon: plasmid unnamed
• Accession: NZ_CP027324
• Organism: Escherichia coli strain 2013C-3033

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C6N24_RS28675
• Protein ID: WP_089611717.1
• Coordinates: 4490..4837 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6N24_RS28670
• Protein ID: WP_089611719.1
• Coordinates: 4191..4490 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N24_RS28645	1164..1805	+	642	WP_024221658.1	recombinase family protein	-
C6N24_RS28650	1854..2651	-	798	Protein_2	transposase	-
C6N24_RS28655	2651..3016	-	366	WP_089611745.1	transposase	-
C6N24_RS28660	3478..3609	-	132	WP_106894632.1	colicin 10 lysis protein	-
C6N24_RS28665	3634..3924	+	291	WP_089611770.1	Cki family colicin immunity protein	-
C6N24_RS28670	4191..4490	-	300	WP_089611719.1	XRE family transcriptional regulator	Antitoxin
C6N24_RS28675	4490..4837	-	348	WP_089611717.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6N24_RS28685	5250..6116	-	867	WP_106894634.1	DMT family transporter	-
C6N24_RS28690	6338..7546	-	1209	WP_106894635.1	IS91 family transposase	-
C6N24_RS28695	7527..7799	-	273	WP_000019163.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..127667	127667
	-	inside	IScluster/Tn	-	-	1227..9288	8061

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13530.45 Da
• Isoelectric Point: 8.8413

>T97980 WP_089611717.1 NZ_CP027324:c4837-4490 [Escherichia coli]
MWTVLFSQRFDNWLNEQEDALQEKVLADLKNLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T97980 NZ_CP027324:c4837-4490 [Escherichia coli]
ATGTGGACGGTACTTTTTAGTCAGCGATTTGACAACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAATCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCCATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAGCGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11042.86 Da
• Isoelectric Point: 5.2090

>AT97980 WP_089611719.1 NZ_CP027324:c4490-4191 [Escherichia coli]
MRTLDEVIASRSPESQARIKEIADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRVAFNI

Nucleotide

• Length: 300 bp

>AT97980 NZ_CP027324:c4490-4191 [Escherichia coli]
ATGAGAACATTAGATGAGGTGATTGCCAGCCGTTCACCTGAAAGCCAGGCGCGAATTAAAGAAATAGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAGGTAGCTGAAGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTGAAGCTGGCGACGTTAAAGCGTTACGTTGAAGCTATGGGG
GGAAAGTTGAGCCTGGATGTTGAACTCCCTACCGGAAAACGAGTGGCCTTTAACATTTAG