Detailed information of TA system TA097977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4360623..4361225
• Replicon: chromosome
• Accession: NZ_CP027323
• Organism: Escherichia coli strain 2013C-3033

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6N24_RS23135
• Protein ID: WP_000897305.1
• Coordinates: 4360623..4360934 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6N24_RS23140
• Protein ID: WP_000356397.1
• Coordinates: 4360935..4361225 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N24_RS23110	4356528..4357127	+	600	WP_001295269.1	glucose-1-phosphatase	-
C6N24_RS23115	4357121..4357993	+	873	WP_000920762.1	virulence factor BrkB family protein	-
C6N24_RS23120	4357990..4358427	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6N24_RS23125	4358472..4359413	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6N24_RS23130	4359477..4360394	-	918	WP_106894553.1	alpha/beta hydrolase	-
C6N24_RS23135	4360623..4360934	+	312	WP_000897305.1	hypothetical protein	Toxin
C6N24_RS23140	4360935..4361225	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6N24_RS23150	4361811..4362029	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C6N24_RS23155	4362248..4362490	+	243	WP_001087409.1	hypothetical protein	-
C6N24_RS23160	4362820..4363749	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C6N24_RS23165	4363746..4364381	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C6N24_RS23170	4364378..4365280	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T97977 WP_000897305.1 NZ_CP027323:4360623-4360934 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T97977 NZ_CP027323:4360623-4360934 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT97977 WP_000356397.1 NZ_CP027323:4360935-4361225 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT97977 NZ_CP027323:4360935-4361225 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7