Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2087561..2088063 Replicon chromosome
Accession NZ_CP027323
Organism Escherichia coli strain 2013C-3033

Toxin (Protein)


Gene name yoeB Uniprot ID D8A584
Locus tag C6N24_RS11480 Protein ID WP_000767828.1
Coordinates 2087561..2087815 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C6N24_RS11485 Protein ID WP_001259255.1
Coordinates 2087812..2088063 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N24_RS11450 2082576..2082803 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
C6N24_RS11455 2082817..2083875 - 1059 WP_000492344.1 transport protein YeeE -
C6N24_RS11460 2084054..2085412 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C6N24_RS11465 2085402..2085464 - 63 WP_010723108.1 membrane protein YoeI -
C6N24_RS11470 2085679..2086608 - 930 WP_000803351.1 LysR family transcriptional regulator -
C6N24_RS11475 2086654..2087478 - 825 WP_000754758.1 SDR family oxidoreductase -
C6N24_RS11480 2087561..2087815 - 255 WP_000767828.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C6N24_RS11485 2087812..2088063 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C6N24_RS11490 2088346..2088396 + 51 WP_001364200.1 his operon leader peptide -
C6N24_RS11500 2088542..2089441 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C6N24_RS11505 2089447..2090751 + 1305 WP_001493159.1 histidinol dehydrogenase -
C6N24_RS11510 2090748..2091818 + 1071 WP_001564244.1 histidinol-phosphate transaminase -
C6N24_RS11515 2091818..2092885 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.56 Da        Isoelectric Point: 8.0353

>T97969 WP_000767828.1 NZ_CP027323:c2087815-2087561 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T97969 NZ_CP027323:c2087815-2087561 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGATCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT97969 WP_001259255.1 NZ_CP027323:c2088063-2087812 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT97969 NZ_CP027323:c2088063-2087812 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G465

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References