Detailed information of TA system TA097968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2040071..2040633
• Replicon: chromosome
• Accession: NZ_CP027323
• Organism: Escherichia coli strain 2013C-3033

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A854AHW2
• Locus tag: C6N24_RS11190
• Protein ID: WP_073568588.1
• Coordinates: 2040071..2040349 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6N24_RS11195
• Protein ID: WP_106894371.1
• Coordinates: 2040349..2040633 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N24_RS11160	2035758..2036816	-	1059	WP_106894376.1	site-specific DNA-methyltransferase	-
C6N24_RS11165	2036967..2037164	-	198	WP_000917768.1	hypothetical protein	-
C6N24_RS11170	2037392..2038213	-	822	WP_106894375.1	antitermination protein	-
C6N24_RS11175	2038210..2038584	-	375	WP_106894374.1	RusA family crossover junction endodeoxyribonuclease	-
C6N24_RS11180	2038597..2039646	-	1050	WP_106894373.1	DUF968 domain-containing protein	-
C6N24_RS11185	2039648..2039920	-	273	WP_106894372.1	hypothetical protein	-
C6N24_RS11190	2040071..2040349	+	279	WP_073568588.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6N24_RS11195	2040349..2040633	+	285	WP_106894371.1	HigA family addiction module antidote protein	Antitoxin
C6N24_RS11200	2040753..2041562	-	810	WP_106894370.1	DUF551 domain-containing protein	-
C6N24_RS11205	2041559..2041768	-	210	WP_032313438.1	hypothetical protein	-
C6N24_RS11210	2041769..2042215	-	447	WP_106894369.1	hypothetical protein	-
C6N24_RS11215	2042352..2042657	-	306	WP_106894368.1	hypothetical protein	-
C6N24_RS11220	2042650..2042877	-	228	WP_106894367.1	hypothetical protein	-
C6N24_RS11225	2042874..2043155	-	282	WP_106894618.1	DNA-binding protein	-
C6N24_RS11230	2043188..2043904	-	717	WP_106894459.1	DUF1627 domain-containing protein	-
C6N24_RS11235	2043934..2044674	-	741	WP_106894460.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2001450..2054776	53326

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10721.34 Da
• Isoelectric Point: 9.9106

>T97968 WP_073568588.1 NZ_CP027323:2040071-2040349 [Escherichia coli]
MIKSFKHKGLKKYYTKGDTSGLEQSLIPRLKNRLSMLDAAKTIQFLDLPGYRLHELKGDRANTWSITVSGNWRLTFIFEN
GDVYILNLEDYH

Nucleotide

• Length: 279 bp

>T97968 NZ_CP027323:2040071-2040349 [Escherichia coli]
ATGATAAAAAGCTTCAAACATAAAGGACTGAAAAAATATTATACGAAAGGAGATACCAGTGGCCTTGAGCAATCTCTGAT
CCCTCGCCTGAAAAACAGATTATCCATGCTGGACGCAGCCAAAACTATTCAGTTCCTTGATCTCCCCGGATACCGGTTAC
ATGAATTAAAAGGCGACCGGGCCAACACGTGGTCAATCACCGTATCAGGAAACTGGCGTCTGACATTCATCTTTGAAAAC
GGTGATGTGTACATACTTAATCTTGAGGACTATCACTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10160.82 Da
• Isoelectric Point: 8.6596

>AT97968 WP_106894371.1 NZ_CP027323:2040349-2040633 [Escherichia coli]
MVMFNPSSPGEIVAEILEDGKVSLRKFATAMDIAPSTASRFLNGKVAVTPEMAMKLAVVIGGDAESWLSLQNNYSLAQAR
KKIDVSHLHRLAFA

Nucleotide

• Length: 285 bp

>AT97968 NZ_CP027323:2040349-2040633 [Escherichia coli]
ATGGTTATGTTTAATCCCTCATCTCCAGGTGAAATCGTCGCTGAGATTCTGGAAGACGGAAAAGTCAGCCTCCGAAAATT
CGCCACAGCAATGGATATTGCGCCATCAACAGCCAGCAGATTTCTTAATGGAAAAGTGGCTGTTACTCCTGAAATGGCAA
TGAAACTCGCAGTCGTGATTGGAGGAGATGCCGAAAGCTGGCTGTCTCTTCAGAACAACTACAGTCTCGCGCAAGCCCGC
AAAAAAATTGATGTAAGTCATCTTCATCGTCTGGCTTTTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A854AHW2