Detailed information of TA system TA097959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 994873..995435
• Replicon: chromosome
• Accession: NZ_CP027323
• Organism: Escherichia coli strain 2013C-3033

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A854AHW2
• Locus tag: C6N24_RS05370
• Protein ID: WP_073568588.1
• Coordinates: 995157..995435 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6N24_RS05365
• Protein ID: WP_106894371.1
• Coordinates: 994873..995157 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N24_RS05325	990832..991572	+	741	WP_096852373.1	ATP-binding protein	-
C6N24_RS05330	991602..992318	+	717	WP_096852374.1	DUF1627 domain-containing protein	-
C6N24_RS05335	992351..992632	+	282	WP_106894611.1	DNA-binding protein	-
C6N24_RS05340	992629..992856	+	228	WP_106894367.1	hypothetical protein	-
C6N24_RS05345	992849..993154	+	306	WP_106894368.1	hypothetical protein	-
C6N24_RS05350	993291..993737	+	447	WP_106894369.1	hypothetical protein	-
C6N24_RS05355	993738..993947	+	210	WP_032313438.1	hypothetical protein	-
C6N24_RS05360	993944..994753	+	810	WP_106894370.1	DUF551 domain-containing protein	-
C6N24_RS05365	994873..995157	-	285	WP_106894371.1	HigA family addiction module antidote protein	Antitoxin
C6N24_RS05370	995157..995435	-	279	WP_073568588.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6N24_RS05375	995586..995858	+	273	WP_106894372.1	hypothetical protein	-
C6N24_RS05380	995860..996909	+	1050	WP_106894373.1	DUF968 domain-containing protein	-
C6N24_RS05385	996922..997296	+	375	WP_106894374.1	RusA family crossover junction endodeoxyribonuclease	-
C6N24_RS05390	997293..998114	+	822	WP_106894375.1	antitermination protein	-
C6N24_RS05395	998342..998539	+	198	WP_000917768.1	hypothetical protein	-
C6N24_RS05400	998690..999748	+	1059	WP_106894376.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	977145..1034170	57025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10721.34 Da
• Isoelectric Point: 9.9106

>T97959 WP_073568588.1 NZ_CP027323:c995435-995157 [Escherichia coli]
MIKSFKHKGLKKYYTKGDTSGLEQSLIPRLKNRLSMLDAAKTIQFLDLPGYRLHELKGDRANTWSITVSGNWRLTFIFEN
GDVYILNLEDYH

Nucleotide

• Length: 279 bp

>T97959 NZ_CP027323:c995435-995157 [Escherichia coli]
ATGATAAAAAGCTTCAAACATAAAGGACTGAAAAAATATTATACGAAAGGAGATACCAGTGGCCTTGAGCAATCTCTGAT
CCCTCGCCTGAAAAACAGATTATCCATGCTGGACGCAGCCAAAACTATTCAGTTCCTTGATCTCCCCGGATACCGGTTAC
ATGAATTAAAAGGCGACCGGGCCAACACGTGGTCAATCACCGTATCAGGAAACTGGCGTCTGACATTCATCTTTGAAAAC
GGTGATGTGTACATACTTAATCTTGAGGACTATCACTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10160.82 Da
• Isoelectric Point: 8.6596

>AT97959 WP_106894371.1 NZ_CP027323:c995157-994873 [Escherichia coli]
MVMFNPSSPGEIVAEILEDGKVSLRKFATAMDIAPSTASRFLNGKVAVTPEMAMKLAVVIGGDAESWLSLQNNYSLAQAR
KKIDVSHLHRLAFA

Nucleotide

• Length: 285 bp

>AT97959 NZ_CP027323:c995157-994873 [Escherichia coli]
ATGGTTATGTTTAATCCCTCATCTCCAGGTGAAATCGTCGCTGAGATTCTGGAAGACGGAAAAGTCAGCCTCCGAAAATT
CGCCACAGCAATGGATATTGCGCCATCAACAGCCAGCAGATTTCTTAATGGAAAAGTGGCTGTTACTCCTGAAATGGCAA
TGAAACTCGCAGTCGTGATTGGAGGAGATGCCGAAAGCTGGCTGTCTCTTCAGAACAACTACAGTCTCGCGCAAGCCCGC
AAAAAAATTGATGTAAGTCATCTTCATCGTCTGGCTTTTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A854AHW2