Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 622397..623048 Replicon chromosome
Accession NZ_CP027323
Organism Escherichia coli strain 2013C-3033

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag C6N24_RS03285 Protein ID WP_032211261.1
Coordinates 622397..622747 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag C6N24_RS03290 Protein ID WP_032211260.1
Coordinates 622749..623048 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N24_RS03260 618076..619836 + 1761 WP_106894341.1 restriction endonuclease subunit S -
C6N24_RS03270 621207..621518 - 312 WP_032211263.1 IS66 family insertion sequence element accessory protein TnpB -
C6N24_RS03275 621515..621946 - 432 WP_159032338.1 transposase -
C6N24_RS03285 622397..622747 + 351 WP_032211261.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6N24_RS03290 622749..623048 + 300 WP_032211260.1 XRE family transcriptional regulator Antitoxin
C6N24_RS03295 623167..623355 + 189 WP_157906239.1 hypothetical protein -
C6N24_RS03300 623698..624348 + 651 WP_106894343.1 IS66-like element accessory protein TnpA -
C6N24_RS03305 624348..624695 + 348 WP_000624622.1 IS66 family insertion sequence element accessory protein TnpB -
C6N24_RS03310 624715..626280 + 1566 WP_106894344.1 IS66 family transposase -
C6N24_RS03320 626569..626889 + 321 WP_000520781.1 ATP-dependent Clp protease adapter ClpS -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 621207..626280 5073
- inside Genomic island - - 593155..623048 29893


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(17-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13437.42 Da        Isoelectric Point: 7.1665

>T97958 WP_032211261.1 NZ_CP027323:622397-622747 [Escherichia coli]
MWNVVTTDLFDAWFMAQNEEIRESVFEAMGLLEKFGPTLGRPYVDTLNGSDFANMKELRVQHKGNPIRAFFAFDPTRNAI
VLCAGDKTGLNEKRFYRDMLKLADSEYRKHLAKLEK

Download         Length: 351 bp

>T97958 NZ_CP027323:622397-622747 [Escherichia coli]
ATGTGGAACGTAGTAACTACAGACCTCTTTGATGCGTGGTTTATGGCGCAAAATGAAGAGATTCGTGAATCAGTGTTTGA
GGCTATGGGATTACTGGAAAAATTTGGGCCAACTTTAGGAAGGCCATATGTAGATACATTGAATGGTTCTGATTTTGCCA
ATATGAAAGAACTCCGCGTTCAGCATAAAGGTAACCCCATCAGAGCATTCTTTGCATTTGATCCTACCCGTAACGCAATT
GTTCTTTGTGCGGGCGATAAAACAGGATTGAACGAGAAGCGTTTCTATAGAGACATGCTCAAACTCGCTGATTCTGAATA
CCGTAAACATCTTGCAAAACTGGAGAAATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11014.75 Da        Isoelectric Point: 8.5545

>AT97958 WP_032211260.1 NZ_CP027323:622749-623048 [Escherichia coli]
MATYRELLAKESPEMQARVAERVEKASLQIALSALRDELNISQTELATMMGVKQPSVARMEQTDNDPRISTLKRYVKALG
GELSLDVTLPTGKRVAFHL

Download         Length: 300 bp

>AT97958 NZ_CP027323:622749-623048 [Escherichia coli]
ATGGCCACCTACAGAGAACTACTGGCTAAAGAGAGTCCTGAAATGCAAGCTCGCGTAGCCGAGCGGGTTGAAAAAGCCAG
CCTTCAGATCGCACTTAGTGCTTTACGTGATGAACTGAACATATCCCAAACCGAACTGGCTACAATGATGGGAGTAAAAC
AACCATCAGTTGCCAGGATGGAACAAACTGACAACGATCCGAGAATATCAACTCTTAAACGTTATGTGAAAGCGTTAGGC
GGTGAGTTAAGTCTGGACGTGACATTGCCTACAGGAAAGCGAGTTGCTTTCCACCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References