Detailed information of TA system TA097944

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2566583..2566844
• Replicon: chromosome
• Accession: NZ_CP027319
• Organism: Escherichia coli strain 2014C-3084

Toxin (Protein)

• Gene name: hokA
• Uniprot ID: V0YDF1
• Locus tag: AAG01_RS12980
• Protein ID: WP_001135738.1
• Coordinates: 2566583..2566735 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	sokA
Locus tag	-
Coordinates	2566810..2566844 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AAG01_RS12950	2562681..2563340	+	660	WP_000747625.1	OmpA family lipoprotein	-
AAG01_RS12955	2563444..2564418	+	975	Protein_2548	glyoxylate/hydroxypyruvate reductase GhrB	-
AAG01_RS12960	2564468..2565178	-	711	WP_000190516.1	DUF3053 domain-containing protein	-
AAG01_RS12965	2565612..2565902	+	291	WP_000455798.1	HTH-type transcriptional regulator	-
AAG01_RS12970	2566183..2566395	+	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
AAG01_RS12975	2566535..2566606	+	72	WP_212738361.1	hypothetical protein	-
AAG01_RS12980	2566583..2566735	-	153	WP_001135738.1	type I toxin-antitoxin system toxin HokA	Toxin
-	2566810..2566844	+	35	-	-	Antitoxin
AAG01_RS12985	2567062..2569131	-	2070	WP_001291788.1	glycine--tRNA ligase subunit beta	-
AAG01_RS12990	2569141..2570052	-	912	WP_001168544.1	glycine--tRNA ligase subunit alpha	-
AAG01_RS12995	2570147..2570446	-	300	WP_000980160.1	YsaB family lipoprotein	-
AAG01_RS13000	2570621..2571616	+	996	WP_001182639.1	O-acetyltransferase WecH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (5-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5912.14 Da
• Isoelectric Point: 7.7173

>T97944 WP_001135738.1 NZ_CP027319:c2566735-2566583 [Escherichia coli]
MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFLACNLKE

Nucleotide

• Length: 153 bp

>T97944 NZ_CP027319:c2566735-2566583 [Escherichia coli]
ATGCCGCAGAAATATAGATTGCTTTCTTTAATAGTGATTTGTTTCACGCTTTTATTTTTCACCTGGATGATAAGAGATTC
ACTGTGTGAATTGCATATTAAACAGGGGAGTTATGAGCTGGCGGCATTTTTAGCCTGCAATTTGAAAGAGTAA

Antitoxin

Nucleotide

• Length: 35 bp

>AT97944 NZ_CP027319:2566810-2566844 [Escherichia coli]
CACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTG

Structures

Toxin

• AlphaFold DB: A0A7U9LX93

Antitoxin