Detailed information of TA system TA097932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1655073..1655800
• Replicon: chromosome
• Accession: NZ_CP027319
• Organism: Escherichia coli strain 2014C-3084

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: AAG01_RS08500
• Protein ID: WP_000547563.1
• Coordinates: 1655489..1655800 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AAG01_RS08495
• Protein ID: WP_000126294.1
• Coordinates: 1655073..1655492 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AAG01_RS08475 (1650122)	1650122..1650649	-	528	WP_001078777.1	electron transport protein HydN	-
AAG01_RS08480 (1650798)	1650798..1651808	-	1011	WP_032161993.1	DNA-binding transcriptional regulator AscG	-
AAG01_RS08485 (1652069)	1652069..1653526	+	1458	WP_001107816.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
AAG01_RS08490 (1653535)	1653535..1654959	+	1425	WP_000110310.1	6-phospho-beta-glucosidase AscB	-
AAG01_RS08495 (1655073)	1655073..1655492	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
AAG01_RS08500 (1655489)	1655489..1655800	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
AAG01_RS08505 (1655962)	1655962..1656717	-	756	WP_001400787.1	hypothetical protein	-
AAG01_RS08510 (1656760)	1656760..1657227	-	468	WP_000132960.1	hydrogenase maturation peptidase HycI	-
AAG01_RS08515 (1657220)	1657220..1657630	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
AAG01_RS08520 (1657627)	1657627..1658394	-	768	WP_000067400.1	formate hydrogenlyase subunit HycG	-
AAG01_RS08525 (1658394)	1658394..1658936	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
AAG01_RS08530 (1658946)	1658946..1660655	-	1710	WP_001288147.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T97932 WP_000547563.1 NZ_CP027319:c1655800-1655489 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T97932 NZ_CP027319:c1655800-1655489 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT97932 WP_000126294.1 NZ_CP027319:c1655492-1655073 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT97932 NZ_CP027319:c1655492-1655073 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACAATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22