Detailed information of TA system TA097930

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 816620..816845
• Replicon: chromosome
• Accession: NZ_CP027319
• Organism: Escherichia coli strain 2014C-3084

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: AAG01_RS04355
• Protein ID: WP_000813258.1
• Coordinates: 816620..816775 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	816787..816845 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AAG01_RS04325	811873..813086	+	1214	WP_136952279.1	IS3 family transposase	-
AAG01_RS04330	813324..813521	-	198	WP_000917763.1	hypothetical protein	-
AAG01_RS04335	813765..814346	-	582	WP_000211416.1	ORF6N domain-containing protein	-
AAG01_RS04340	814620..815174	-	555	WP_000640148.1	DUF1133 family protein	-
AAG01_RS04345	815171..815461	-	291	WP_000228019.1	DUF1364 domain-containing protein	-
AAG01_RS04350	815461..816060	-	600	WP_000940348.1	DUF1367 family protein	-
AAG01_RS04355	816620..816775	-	156	WP_000813258.1	Hok/Gef family protein	Toxin
-	816787..816845	+	59	-	-	Antitoxin
AAG01_RS04360	817021..817125	-	105	WP_001278450.1	hypothetical protein	-
AAG01_RS04365	817241..817594	-	354	WP_000610382.1	DUF551 domain-containing protein	-
AAG01_RS04370	817591..817914	-	324	WP_044788429.1	hypothetical protein	-
AAG01_RS04375	817999..818211	-	213	WP_000063625.1	hypothetical protein	-
AAG01_RS04380	818260..818616	-	357	WP_000403779.1	hypothetical protein	-
AAG01_RS04385	818674..819069	-	396	WP_001118155.1	DUF977 family protein	-
AAG01_RS04390	819085..819855	-	771	WP_032313847.1	DUF1627 domain-containing protein	-
AAG01_RS04395	819877..820623	-	747	WP_000788990.1	ATP-binding protein	-
AAG01_RS04400	820630..821498	-	869	Protein_871	DUF1376 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	775632..838859	63227

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T97930 WP_000813258.1 NZ_CP027319:c816775-816620 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T97930 NZ_CP027319:c816775-816620 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACAGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT97930 NZ_CP027319:816787-816845 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin