Detailed information of TA system TA097927

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 548783..549008
• Replicon: chromosome
• Accession: NZ_CP027319
• Organism: Escherichia coli strain 2014C-3084

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: AAG01_RS03000
• Protein ID: WP_000813254.1
• Coordinates: 548853..549008 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	548783..548841 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AAG01_RS02940	544123..544515	+	393	WP_001141097.1	DUF977 family protein	-
AAG01_RS02945	544512..544808	+	297	WP_001266133.1	DUF4406 domain-containing protein	-
AAG01_RS02950	544805..545180	+	376	Protein_584	ead/Ea22-like family protein	-
AAG01_RS02955	545158..545514	+	357	WP_000403783.1	hypothetical protein	-
AAG01_RS02960	545565..545777	+	213	WP_000935422.1	hypothetical protein	-
AAG01_RS02965	545811..545993	+	183	WP_001224662.1	hypothetical protein	-
AAG01_RS02970	545986..546162	+	177	WP_000753069.1	hypothetical protein	-
AAG01_RS24000	546159..546494	+	336	Protein_589	ead/Ea22-like family protein	-
AAG01_RS02980	546856..547212	+	357	WP_000403779.1	hypothetical protein	-
AAG01_RS02985	547261..547473	+	213	WP_000063625.1	hypothetical protein	-
AAG01_RS02990	547674..548387	+	714	WP_000555106.1	DUF551 domain-containing protein	-
AAG01_RS02995	548503..548607	+	105	WP_001278450.1	hypothetical protein	-
-	548783..548841	-	59	-	-	Antitoxin
AAG01_RS03000	548853..549008	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
AAG01_RS03005	549111..549428	-	318	WP_000042395.1	DNA-binding transcriptional regulator	-
AAG01_RS03010	549421..549792	-	372	WP_001217944.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AAG01_RS03015	550049..550243	+	195	WP_000701093.1	hypothetical protein	-
AAG01_RS03020	550297..550566	+	270	WP_012817785.1	hypothetical protein	-
AAG01_RS03025	550568..551617	+	1050	WP_106873513.1	DUF968 domain-containing protein	-
AAG01_RS03030	551631..552383	+	753	WP_001047111.1	antitermination protein	-
AAG01_RS03035	552665..552844	+	180	WP_250220475.1	site-specific DNA-methyltransferase	-
AAG01_RS03055	553225..553449	+	225	WP_000735807.1	hypothetical protein	-
AAG01_RS03060	553502..553711	+	210	WP_000498121.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	516058..581428	65370

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T97927 WP_000813254.1 NZ_CP027319:548853-549008 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T97927 NZ_CP027319:548853-549008 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGCTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT97927 NZ_CP027319:c548841-548783 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin