Detailed information of TA system TA097910

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3249042..3249644
• Replicon: chromosome
• Accession: NZ_CP027317
• Organism: Escherichia coli strain 2015C-3107

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6N21_RS17230
• Protein ID: WP_000897305.1
• Coordinates: 3249042..3249353 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6N21_RS17235
• Protein ID: WP_000356397.1
• Coordinates: 3249354..3249644 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N21_RS17200	3244086..3244523	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6N21_RS17205	3244568..3245509	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6N21_RS17210	3245873..3246811	+	939	Protein_3177	hypothetical protein	-
C6N21_RS17215	3246824..3247738	+	915	WP_072097029.1	transposase	-
C6N21_RS17225	3247906..3248813	-	908	Protein_3179	alpha/beta hydrolase	-
C6N21_RS17230	3249042..3249353	+	312	WP_000897305.1	hypothetical protein	Toxin
C6N21_RS17235	3249354..3249644	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6N21_RS17245	3250230..3250448	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C6N21_RS17250	3250667..3250909	+	243	WP_001086388.1	hypothetical protein	-
C6N21_RS17255	3251239..3252168	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C6N21_RS17260	3252165..3252800	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C6N21_RS17265	3252797..3253699	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T97910 WP_000897305.1 NZ_CP027317:3249042-3249353 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T97910 NZ_CP027317:3249042-3249353 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT97910 WP_000356397.1 NZ_CP027317:3249354-3249644 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT97910 NZ_CP027317:3249354-3249644 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7