Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1038637..1039139 Replicon chromosome
Accession NZ_CP027317
Organism Escherichia coli strain 2015C-3107

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C6N21_RS05755 Protein ID WP_000767829.1
Coordinates 1038637..1038891 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C6N21_RS05760 Protein ID WP_001259255.1
Coordinates 1038888..1039139 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N21_RS05725 1033652..1033879 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
C6N21_RS05730 1033893..1034951 - 1059 WP_000492305.1 transport protein YeeE -
C6N21_RS05735 1035130..1036488 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C6N21_RS05740 1036478..1036540 - 63 WP_010723108.1 membrane protein YoeI -
C6N21_RS05745 1036755..1037684 - 930 WP_000803351.1 LysR family transcriptional regulator -
C6N21_RS05750 1037730..1038554 - 825 WP_000754721.1 SDR family oxidoreductase -
C6N21_RS05755 1038637..1038891 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C6N21_RS05760 1038888..1039139 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C6N21_RS05765 1039422..1039472 + 51 WP_001364200.1 his operon leader peptide -
C6N21_RS05775 1039618..1040517 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C6N21_RS05780 1040523..1041827 + 1305 WP_000009587.1 histidinol dehydrogenase -
C6N21_RS05785 1041824..1042894 + 1071 WP_000108943.1 histidinol-phosphate transaminase -
C6N21_RS05790 1042894..1043961 + 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T97899 WP_000767829.1 NZ_CP027317:c1038891-1038637 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T97899 NZ_CP027317:c1038891-1038637 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT97899 WP_001259255.1 NZ_CP027317:c1039139-1038888 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT97899 NZ_CP027317:c1039139-1038888 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References