Detailed information of TA system TA097886

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 177595..177966
• Replicon: chromosome
• Accession: NZ_CP027317
• Organism: Escherichia coli strain 2015C-3107

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: C6N21_RS01010
• Protein ID: WP_001317028.1
• Coordinates: 177772..177966 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	177595..177773 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N21_RS00980	173347..173520	+	174	WP_001296046.1	hypothetical protein	-
C6N21_RS00985	173550..174923	+	1374	WP_000123758.1	ATP-dependent RNA helicase DbpA	-
C6N21_RS00990	175052..175987	-	936	WP_001157421.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
C6N21_RS00995	176039..177274	-	1236	WP_000040852.1	site-specific integrase	-
C6N21_RS01000	177276..177491	-	216	WP_000079604.1	excisionase XisR	-
C6N21_RS01005	177570..177779	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	177595..177773	+	179	NuclAT_0	-	Antitoxin
-	177595..177773	+	179	NuclAT_0	-	Antitoxin
-	177595..177773	+	179	NuclAT_0	-	Antitoxin
-	177595..177773	+	179	NuclAT_0	-	Antitoxin
C6N21_RS01010	177772..177966	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
C6N21_RS01015	178023..178832	-	810	WP_000595429.1	recombination protein RecT	-
C6N21_RS01020	178825..181494	-	2670	WP_000102191.1	exodeoxyribonuclease VIII	-
C6N21_RS01025	181575..181751	-	177	WP_000245534.1	hypothetical protein	-
C6N21_RS01030	181745..181966	-	222	WP_000560223.1	killing protein KilR	-
C6N21_RS01035	182391..182543	-	153	WP_001169149.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	176039..286796	110757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T97886 WP_001317028.1 NZ_CP027317:c177966-177772 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T97886 NZ_CP027317:c177966-177772 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT97886 NZ_CP027317:177595-177773 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin