Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4280616..4281415 Replicon chromosome
Accession NZ_CP027312
Organism Escherichia coli strain 2013C-3181

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag MJ73_RS22145 Protein ID WP_000347266.1
Coordinates 4280616..4281080 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MJ73_RS22150 Protein ID WP_001307405.1
Coordinates 4281080..4281415 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ73_RS22110 4275617..4276051 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
MJ73_RS22115 4276069..4276947 - 879 WP_001315856.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
MJ73_RS22120 4276937..4277716 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
MJ73_RS22125 4277727..4278200 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
MJ73_RS22130 4278223..4279503 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
MJ73_RS22140 4279752..4280561 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MJ73_RS22145 4280616..4281080 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MJ73_RS22150 4281080..4281415 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MJ73_RS22155 4281564..4283135 - 1572 WP_001273741.1 galactarate dehydratase -
MJ73_RS22160 4283510..4284844 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
MJ73_RS22165 4284860..4285630 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T97881 WP_000347266.1 NZ_CP027312:c4281080-4280616 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T97881 NZ_CP027312:c4281080-4280616 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT97881 WP_001307405.1 NZ_CP027312:c4281415-4281080 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT97881 NZ_CP027312:c4281415-4281080 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References