Detailed information of TA system TA097878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3422511..3423113
• Replicon: chromosome
• Accession: NZ_CP027312
• Organism: Escherichia coli strain 2013C-3181

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: MJ73_RS17700
• Protein ID: WP_000897305.1
• Coordinates: 3422802..3423113 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MJ73_RS17695
• Protein ID: WP_000356397.1
• Coordinates: 3422511..3422801 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ73_RS17665	3418437..3419339	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
MJ73_RS17670	3419336..3419971	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
MJ73_RS17675	3419968..3420897	+	930	WP_106918627.1	formate dehydrogenase accessory protein FdhE	-
MJ73_RS17680	3421227..3421469	-	243	WP_001086388.1	hypothetical protein	-
MJ73_RS17685	3421688..3421906	-	219	WP_001297075.1	ribbon-helix-helix domain-containing protein	-
MJ73_RS17695	3422511..3422801	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
MJ73_RS17700	3422802..3423113	-	312	WP_000897305.1	hypothetical protein	Toxin
MJ73_RS17705	3423342..3424250	+	909	WP_001162704.1	alpha/beta hydrolase	-
MJ73_RS17710	3424314..3425255	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
MJ73_RS17715	3425300..3425737	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
MJ73_RS17720	3425734..3426606	-	873	WP_000920762.1	virulence factor BrkB family protein	-
MJ73_RS17725	3426600..3427199	-	600	WP_001315111.1	glucose-1-phosphatase	-
MJ73_RS17730	3427298..3428083	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T97878 WP_000897305.1 NZ_CP027312:c3423113-3422802 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T97878 NZ_CP027312:c3423113-3422802 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT97878 WP_000356397.1 NZ_CP027312:c3422801-3422511 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT97878 NZ_CP027312:c3422801-3422511 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7