Detailed information of TA system TA097857

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2746..3171
• Replicon: plasmid unnamed
• Accession: NZ_CP027311
• Organism: Escherichia coli strain 2014C-4135

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: C6N22_RS25495
• Protein ID: WP_001332444.1
• Coordinates: 3010..3171 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	2746..2957 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N22_RS25485	1584..2018	+	435	WP_000845873.1	conjugation system SOS inhibitor PsiB	-
C6N22_RS25490	2015..2734	+	720	WP_044863095.1	plasmid SOS inhibition protein A	-
-	2746..2957	+	212	NuclAT_0	-	Antitoxin
-	2746..2957	+	212	NuclAT_0	-	Antitoxin
-	2746..2957	+	212	NuclAT_0	-	Antitoxin
-	2746..2957	+	212	NuclAT_0	-	Antitoxin
C6N22_RS25495	3010..3171	+	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
C6N22_RS26630	3472..3750	-	279	Protein_4	hypothetical protein	-
C6N22_RS25515	4157..4354	+	198	WP_170923508.1	hypothetical protein	-
C6N22_RS25520	4473..5294	+	822	WP_044862944.1	DUF945 domain-containing protein	-
C6N22_RS25525	5590..6180	-	591	WP_199852244.1	transglycosylase SLT domain-containing protein	-
C6N22_RS25530	6514..6897	+	384	WP_044862945.1	relaxosome protein TraM	-
C6N22_RS25535	7084..7773	+	690	WP_044862946.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP	1..133438	133438

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T97857 WP_001332444.1 NZ_CP027311:3010-3171 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T97857 NZ_CP027311:3010-3171 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 212 bp

>AT97857 NZ_CP027311:2746-2957 [Escherichia coli]
TCACACGGATTACCCGTGAACTGGTTGAACGACCAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin