Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4184393..4184895 Replicon chromosome
Accession NZ_CP027310
Organism Escherichia coli strain 2014C-4135

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C6N22_RS21620 Protein ID WP_000767829.1
Coordinates 4184393..4184647 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C6N22_RS21625 Protein ID WP_001259255.1
Coordinates 4184644..4184895 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N22_RS21590 4179408..4179635 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
C6N22_RS21595 4179649..4180707 - 1059 WP_000492346.1 transport protein YeeE -
C6N22_RS21600 4180886..4182244 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C6N22_RS21605 4182234..4182296 - 63 WP_010723108.1 membrane protein YoeI -
C6N22_RS21610 4182511..4183440 - 930 WP_000803351.1 LysR family transcriptional regulator -
C6N22_RS21615 4183486..4184310 - 825 WP_000754749.1 SDR family oxidoreductase -
C6N22_RS21620 4184393..4184647 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C6N22_RS21625 4184644..4184895 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C6N22_RS21630 4185178..4185228 + 51 WP_001364200.1 his operon leader peptide -
C6N22_RS21640 4185374..4186273 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C6N22_RS21645 4186279..4187583 + 1305 WP_001369878.1 histidinol dehydrogenase -
C6N22_RS21650 4187580..4188650 + 1071 WP_000108962.1 histidinol-phosphate transaminase -
C6N22_RS21655 4188650..4189717 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T97855 WP_000767829.1 NZ_CP027310:c4184647-4184393 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T97855 NZ_CP027310:c4184647-4184393 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT97855 WP_001259255.1 NZ_CP027310:c4184895-4184644 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT97855 NZ_CP027310:c4184895-4184644 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References