Detailed information of TA system TA097838

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1138109..1138711
• Replicon: chromosome
• Accession: NZ_CP027310
• Organism: Escherichia coli strain 2014C-4135

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6N22_RS05825
• Protein ID: WP_000897305.1
• Coordinates: 1138400..1138711 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6N22_RS05820
• Protein ID: WP_000356397.1
• Coordinates: 1138109..1138399 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N22_RS05790	1134035..1134937	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
C6N22_RS05795	1134934..1135569	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C6N22_RS05800	1135566..1136495	+	930	WP_000027706.1	formate dehydrogenase accessory protein FdhE	-
C6N22_RS05805	1136825..1137067	-	243	WP_001087409.1	hypothetical protein	-
C6N22_RS05810	1137286..1137504	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C6N22_RS05820	1138109..1138399	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6N22_RS05825	1138400..1138711	-	312	WP_000897305.1	hypothetical protein	Toxin
C6N22_RS05830	1138940..1139848	+	909	WP_001162704.1	alpha/beta hydrolase	-
C6N22_RS05835	1139912..1140853	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6N22_RS05840	1140898..1141335	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6N22_RS05845	1141332..1142204	-	873	WP_000920762.1	virulence factor BrkB family protein	-
C6N22_RS05850	1142198..1142797	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T97838 WP_000897305.1 NZ_CP027310:c1138711-1138400 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T97838 NZ_CP027310:c1138711-1138400 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT97838 WP_000356397.1 NZ_CP027310:c1138399-1138109 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT97838 NZ_CP027310:c1138399-1138109 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7