Detailed information of TA system TA097832

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 47127..47838
• Replicon: plasmid unnamed2
• Accession: NZ_CP027309
• Organism: Escherichia coli strain 2015C-3108

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: C6N27_RS29445
• Protein ID: WP_000162415.1
• Coordinates: 47536..47838 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6N27_RS29440
• Protein ID: WP_000806445.1
• Coordinates: 47127..47465 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N27_RS29410	42421..43320	-	900	WP_032271984.1	hypothetical protein	-
C6N27_RS29415	43589..44242	+	654	WP_023908597.1	hypothetical protein	-
C6N27_RS29420	44571..44900	+	330	WP_000542383.1	hypothetical protein	-
C6N27_RS29425	44893..46086	+	1194	WP_032271983.1	hypothetical protein	-
C6N27_RS29430	46120..46848	-	729	WP_000986754.1	hypothetical protein	-
C6N27_RS29440	47127..47465	-	339	WP_000806445.1	HigA family addiction module antidote protein	Antitoxin
C6N27_RS29445	47536..47838	-	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6N27_RS29450	48001..48792	+	792	WP_000532305.1	hypothetical protein	-
C6N27_RS29455	48789..49556	+	768	WP_106895324.1	baseplate	-
C6N27_RS29460	49560..50540	+	981	WP_000046499.1	hypothetical protein	-
C6N27_RS29465	50537..51190	+	654	WP_000828873.1	hypothetical protein	-
C6N27_RS29470	51250..52155	+	906	WP_001258014.1	recombination-associated protein RdgC	-
C6N27_RS29475	52169..52819	+	651	WP_032153356.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93724	93724

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T97832 WP_000162415.1 NZ_CP027309:c47838-47536 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T97832 NZ_CP027309:c47838-47536 [Escherichia coli]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT97832 WP_000806445.1 NZ_CP027309:c47465-47127 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT97832 NZ_CP027309:c47465-47127 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3