Detailed information of TA system TA097824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4118808..4119410
• Replicon: chromosome
• Accession: NZ_CP027307
• Organism: Escherichia coli strain 2015C-3108

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6N27_RS22240
• Protein ID: WP_000897305.1
• Coordinates: 4119099..4119410 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6N27_RS22235
• Protein ID: WP_000356397.1
• Coordinates: 4118808..4119098 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N27_RS22205	4114733..4115635	+	903	WP_000331369.1	formate dehydrogenase O subunit beta	-
C6N27_RS22210	4115632..4116267	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
C6N27_RS22215	4116264..4117193	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C6N27_RS22220	4117524..4117766	-	243	WP_001086388.1	hypothetical protein	-
C6N27_RS22225	4117985..4118203	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C6N27_RS22235	4118808..4119098	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6N27_RS22240	4119099..4119410	-	312	WP_000897305.1	hypothetical protein	Toxin
C6N27_RS22245	4119639..4120547	+	909	WP_001162704.1	alpha/beta hydrolase	-
C6N27_RS22250	4120611..4121552	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6N27_RS22255	4121597..4122034	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6N27_RS22260	4122031..4122903	-	873	WP_000920762.1	virulence factor BrkB family protein	-
C6N27_RS22265	4122897..4123496	-	600	WP_001315111.1	glucose-1-phosphatase	-
C6N27_RS22270	4123595..4124380	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T97824 WP_000897305.1 NZ_CP027307:c4119410-4119099 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T97824 NZ_CP027307:c4119410-4119099 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT97824 WP_000356397.1 NZ_CP027307:c4119098-4118808 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT97824 NZ_CP027307:c4119098-4118808 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7