Detailed information of TA system TA097775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 1692225..1692892
• Replicon: chromosome
• Accession: NZ_CP027260
• Organism: Pectobacterium parmentieri strain IFB5427

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B3F7E4
• Locus tag: C5E18_RS07970
• Protein ID: WP_033071239.1
• Coordinates: 1692225..1692539 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C5E18_RS07975
• Protein ID: WP_015841345.1
• Coordinates: 1692551..1692892 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5E18_RS07940	1687857..1688468	-	612	WP_033071233.1	phage tail protein I	-
C5E18_RS07945	1688461..1689369	-	909	WP_033071234.1	baseplate assembly protein	-
C5E18_RS07950	1689374..1689718	-	345	WP_033071235.1	GPW/gp25 family protein	-
C5E18_RS07955	1689715..1690350	-	636	WP_033071236.1	phage baseplate assembly protein V	-
C5E18_RS07960	1690457..1691206	+	750	WP_127087869.1	hypothetical protein	-
C5E18_RS07965	1691252..1691881	-	630	WP_033071238.1	ORF6N domain-containing protein	-
C5E18_RS07970	1692225..1692539	+	315	WP_033071239.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C5E18_RS07975	1692551..1692892	+	342	WP_015841345.1	HigA family addiction module antidote protein	Antitoxin
C5E18_RS07980	1692921..1693370	-	450	WP_033071240.1	phage virion morphogenesis protein	-
C5E18_RS07985	1693363..1693818	-	456	WP_033071241.1	phage tail protein	-
C5E18_RS07990	1693908..1694351	-	444	WP_033071242.1	Rz-like lysis system protein LysB	-
C5E18_RS07995	1694348..1694857	-	510	WP_033071243.1	lysozyme	-
C5E18_RS08000	1694841..1695062	-	222	WP_033071244.1	hypothetical protein	-
C5E18_RS08005	1695053..1695256	-	204	WP_033071245.1	tail protein X	-
C5E18_RS08010	1695256..1695759	-	504	WP_033071246.1	head completion/stabilization protein	-
C5E18_RS08015	1695851..1696510	-	660	WP_033071247.1	terminase endonuclease subunit	-
C5E18_RS08020	1696514..1697629	-	1116	WP_033071248.1	phage major capsid protein, P2 family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12183.99 Da
• Isoelectric Point: 10.1205

>T97775 WP_033071239.1 NZ_CP027260:1692225-1692539 [Pectobacterium parmentieri]
MQKMRSIHSFRDKWLEDFFMYGKSHKKIPADVVTSLSRKLDIINAAVSAKDLRSPPGNRYENLNPPLGEYSSIRVNIQYR
LIFKWVDGKAIDVYLDDHGYKNHK

Nucleotide

• Length: 315 bp

>T97775 NZ_CP027260:1692225-1692539 [Pectobacterium parmentieri]
ATGCAAAAAATGCGCAGCATTCATAGCTTTCGGGATAAGTGGCTGGAAGATTTTTTTATGTATGGTAAGTCGCATAAAAA
GATACCTGCCGATGTGGTTACATCCTTGTCCCGCAAGCTGGATATCATCAACGCGGCGGTATCGGCAAAAGATCTGAGAT
CGCCACCGGGGAACCGATACGAGAATCTCAACCCACCATTGGGGGAATACTCATCCATCAGAGTGAATATCCAATATCGC
CTGATTTTTAAATGGGTTGATGGTAAAGCGATTGATGTTTATCTGGATGACCACGGTTACAAAAATCATAAGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12941.56 Da
• Isoelectric Point: 4.4346

>AT97775 WP_015841345.1 NZ_CP027260:1692551-1692892 [Pectobacterium parmentieri]
MAQAQRKPTSVGDVLLYEYLEPTGLKINDLAEMLNVHRNTISALVNNNRKLTVDMAFRLAKAFDTSVDFWLNLQNSVDVW
EVENDARTQEEISRITTLENFLAQRDENVKDVA

Nucleotide

• Length: 342 bp

>AT97775 NZ_CP027260:1692551-1692892 [Pectobacterium parmentieri]
ATGGCACAGGCACAACGCAAGCCGACATCCGTAGGCGATGTTCTGTTGTATGAATATCTTGAACCGACGGGGTTGAAGAT
TAATGACCTGGCGGAAATGCTGAACGTCCACCGCAATACGATCAGCGCATTGGTCAACAACAACCGTAAGCTAACGGTTG
ATATGGCTTTCCGGTTGGCGAAAGCGTTTGATACATCAGTTGATTTCTGGCTTAACCTGCAAAATAGTGTTGACGTATGG
GAAGTGGAAAATGACGCGCGCACTCAGGAAGAAATTAGCCGGATCACCACGCTGGAAAATTTTCTGGCGCAGCGCGATGA
AAATGTGAAAGACGTCGCATAA

Structures

Toxin

• AlphaFold DB: A0A8B3F7E4