Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 430956..431674 Replicon chromosome
Accession NZ_CP027260
Organism Pectobacterium parmentieri strain IFB5427

Toxin (Protein)


Gene name higB Uniprot ID A0A8B3FNM7
Locus tag C5E18_RS02040 Protein ID WP_033070820.1
Coordinates 430956..431261 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B3FM67
Locus tag C5E18_RS02045 Protein ID WP_033070769.1
Coordinates 431258..431674 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5E18_RS02010 426311..427045 + 735 WP_012822189.1 type I-E CRISPR-associated protein Cas5/CasD -
C5E18_RS02015 427045..427692 + 648 WP_012822190.1 type I-E CRISPR-associated protein Cas6/Cse3/CasE -
C5E18_RS02020 427720..428637 + 918 WP_012822191.1 type I-E CRISPR-associated endonuclease Cas1 -
C5E18_RS02025 428638..428931 + 294 WP_005973730.1 type I-E CRISPR-associated endoribonuclease Cas2 -
C5E18_RS02030 430099..430470 - 372 WP_012822192.1 helix-turn-helix domain-containing protein -
C5E18_RS02035 430544..430804 - 261 WP_012822193.1 type II toxin-antitoxin system RelE/ParE family toxin -
C5E18_RS02040 430956..431261 + 306 WP_033070820.1 type II toxin-antitoxin system HigB family toxin Toxin
C5E18_RS02045 431258..431674 + 417 WP_033070769.1 helix-turn-helix domain-containing protein Antitoxin
C5E18_RS02050 432833..433291 + 459 WP_012822197.1 hypothetical protein -
C5E18_RS02055 433372..434724 - 1353 WP_033070770.1 aspartate aminotransferase family protein -
C5E18_RS02060 434755..436026 - 1272 WP_033070821.1 MFS transporter -
C5E18_RS02065 436181..436627 + 447 WP_012822200.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(18-90)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 12010.78 Da        Isoelectric Point: 10.0386

>T97771 WP_033070820.1 NZ_CP027260:430956-431261 [Pectobacterium parmentieri]
VFSRAPFDTAIAQFPNQAAALADLYRTIKREIYATPDDMKKRFPSLDRMKYREKWWVIDIGGGHLRVMFFADFERGKIFI
KHITSHAEYGSLTEYYRRNKE

Download         Length: 306 bp

>T97771 NZ_CP027260:430956-431261 [Pectobacterium parmentieri]
GTGTTCTCCCGGGCACCTTTTGACACTGCAATCGCCCAATTCCCTAATCAGGCGGCAGCACTGGCTGACCTATACCGGAC
GATAAAGCGTGAAATATATGCAACGCCGGACGATATGAAAAAACGTTTCCCCAGCTTAGACAGGATGAAGTACAGGGAAA
AGTGGTGGGTTATTGATATTGGTGGTGGGCATCTTCGGGTGATGTTTTTCGCTGATTTTGAGCGAGGGAAAATTTTTATC
AAACACATCACATCCCATGCAGAGTACGGCAGTCTGACAGAATATTACCGGAGGAATAAAGAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15233.53 Da        Isoelectric Point: 4.3708

>AT97771 WP_033070769.1 NZ_CP027260:431258-431674 [Pectobacterium parmentieri]
MMYADAIKAANNLTSIVPLLGGSTSCKDYEDALKLIEYLLESEPDSPLIDMLTAKIDKYEEDAPEFVAFNERIKALPAGI
ALLRILMDQHGLTQSDFEAEIGKKSLVSRILNGERTLTVDHMSALAKRFDIPVSAFVD

Download         Length: 417 bp

>AT97771 NZ_CP027260:431258-431674 [Pectobacterium parmentieri]
ATGATGTACGCAGACGCAATCAAGGCCGCTAACAATCTGACGAGTATCGTACCGCTCCTCGGAGGTAGCACCTCTTGTAA
AGATTATGAGGATGCGCTGAAACTCATTGAATATTTACTCGAGAGCGAGCCTGATAGCCCGCTAATCGATATGCTGACGG
CTAAAATTGACAAATATGAAGAGGATGCGCCAGAGTTTGTAGCTTTCAACGAGCGTATTAAAGCGCTCCCTGCTGGTATC
GCTTTGCTGCGCATACTTATGGATCAGCATGGGCTAACGCAAAGTGACTTTGAAGCTGAGATCGGTAAAAAATCTCTGGT
ATCCCGAATTCTAAATGGTGAGCGTACACTAACGGTTGATCACATGAGCGCACTTGCTAAACGATTTGATATTCCCGTGA
GTGCTTTTGTTGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3FNM7

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3FM67

References