Detailed information of TA system TA097749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1884868..1885470
• Replicon: chromosome
• Accession: NZ_CP027255
• Organism: Escherichia coli strain EC11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6C13_RS09660
• Protein ID: WP_000897305.1
• Coordinates: 1884868..1885179 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6C13_RS09665
• Protein ID: WP_000356397.1
• Coordinates: 1885180..1885470 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6C13_RS09635	1880782..1881381	+	600	WP_001295269.1	glucose-1-phosphatase	-
C6C13_RS09640	1881375..1882247	+	873	WP_000920762.1	virulence factor BrkB family protein	-
C6C13_RS09645	1882244..1882681	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6C13_RS09650	1882726..1883667	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
C6C13_RS09655	1883731..1884639	-	909	WP_001162704.1	alpha/beta hydrolase	-
C6C13_RS09660	1884868..1885179	+	312	WP_000897305.1	hypothetical protein	Toxin
C6C13_RS09665	1885180..1885470	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6C13_RS09675	1886075..1886293	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C6C13_RS09680	1886512..1886754	+	243	WP_001086388.1	hypothetical protein	-
C6C13_RS09685	1886983..1887963	-	981	WP_000399676.1	IS110-like element IS621 family transposase	-
C6C13_RS09695	1888362..1889291	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C6C13_RS09700	1889288..1889923	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1886983..1887963	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T97749 WP_000897305.1 NZ_CP027255:1884868-1885179 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T97749 NZ_CP027255:1884868-1885179 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT97749 WP_000356397.1 NZ_CP027255:1885180-1885470 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT97749 NZ_CP027255:1885180-1885470 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7