Detailed information of TA system TA097734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 94147..94732
• Replicon: plasmid pOXA58_100004
• Accession: NZ_CP027249
• Organism: Acinetobacter pittii strain WCHAP100004

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: C6N19_RS01030
• Protein ID: WP_000897307.1
• Coordinates: 94412..94732 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6N19_RS01025
• Protein ID: WP_000369781.1
• Coordinates: 94147..94419 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N19_RS01010	90008..91216	+	1209	WP_000343018.1	IS256-like element ISAba26 family transposase	-
C6N19_RS01015	91227..92123	-	897	WP_106405803.1	Mph(E) family macrolide 2'-phosphotransferase	-
C6N19_RS01020	92179..93654	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
C6N19_RS01025	94147..94419	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
C6N19_RS01030	94412..94732	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6N19_RS01035	94872..95291	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
C6N19_RS01040	95371..95652	-	282	WP_000985609.1	putative addiction module antidote protein	-
C6N19_RS01045	95653..95955	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C6N19_RS01050	96104..96634	+	531	WP_000172759.1	hypothetical protein	-
C6N19_RS01055	96723..97037	-	315	WP_004728120.1	BrnA antitoxin family protein	-
C6N19_RS01060	97024..97311	-	288	WP_000438825.1	BrnT family toxin	-
C6N19_RS01065	97541..98146	-	606	WP_000447788.1	LysE family translocator	-
C6N19_RS01070	98196..99023	-	828	WP_000073658.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / floR / sul2 / aph(3'')-Ib / aph(3')-VIb / blaPER-1 / aph(6)-Id / mph(E) / msr(E) / blaOXA-58	-	1..105591	105591

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T97734 WP_000897307.1 NZ_CP027249:c94732-94412 [Acinetobacter pittii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T97734 NZ_CP027249:c94732-94412 [Acinetobacter pittii]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT97734 WP_000369781.1 NZ_CP027249:c94419-94147 [Acinetobacter pittii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT97734 NZ_CP027249:c94419-94147 [Acinetobacter pittii]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3