Detailed information of TA system TA097732

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 30095..30698
• Replicon: plasmid p1_100004
• Accession: NZ_CP027247
• Organism: Acinetobacter pittii strain WCHAP100004

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C6N19_RS00215
• Protein ID: WP_000288004.1
• Coordinates: 30095..30424 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6N19_RS00220
• Protein ID: WP_001258993.1
• Coordinates: 30411..30698 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N19_RS00175	25897..26225	+	329	Protein_33	DUF4236 domain-containing protein	-
C6N19_RS00180	26467..26979	-	513	WP_000464612.1	Fic family protein	-
C6N19_RS00185	26982..27167	-	186	WP_000755724.1	hypothetical protein	-
C6N19_RS00190	27334..28266	+	933	WP_000743263.1	IS5 family transposase	-
C6N19_RS00195	28397..28594	-	198	WP_025464206.1	hypothetical protein	-
C6N19_RS00200	28687..28905	-	219	WP_000994925.1	hypothetical protein	-
C6N19_RS00205	28962..29564	-	603	WP_000096286.1	hypothetical protein	-
C6N19_RS00210	29771..30040	+	270	WP_000453245.1	transcriptional regulator	-
C6N19_RS00215	30095..30424	+	330	WP_000288004.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6N19_RS00220	30411..30698	+	288	WP_001258993.1	helix-turn-helix domain-containing protein	Antitoxin
C6N19_RS00225	30735..30923	-	189	WP_000340067.1	hypothetical protein	-
C6N19_RS00230	31325..31648	-	324	Protein_44	DUF4236 domain-containing protein	-
C6N19_RS00235	31657..31833	-	177	WP_002120129.1	hypothetical protein	-
C6N19_RS20475	31905..32228	-	324	WP_005804845.1	hypothetical protein	-
C6N19_RS00245	32583..33092	-	510	Protein_47	Terminase-like domain protein	-
C6N19_RS00250	33076..33599	-	524	Protein_48	DUF2280 domain-containing protein	-
C6N19_RS00255	33690..34331	-	642	WP_000372120.1	hypothetical protein	-
C6N19_RS00260	34300..34761	-	462	WP_000378528.1	hypothetical protein	-
C6N19_RS00265	34822..35277	-	456	WP_001136766.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..66765	66765
	-	flank	IS/Tn	-	-	27334..28266	932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-90)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12571.69 Da
• Isoelectric Point: 8.8102

>T97732 WP_000288004.1 NZ_CP027247:30095-30424 [Acinetobacter pittii]
MYTIEYYSEDVQDEILTLPDGLLARYFNLTDRMVIYGANLGEPHTLPMSKGLFELRLKSQEGIARVMYCTLVGKRIVMLH
SFVKKTQKTPKQDLNLALDRMKEVKNANT

Nucleotide

• Length: 330 bp

>T97732 NZ_CP027247:30095-30424 [Acinetobacter pittii]
ATGTATACAATTGAATATTACAGCGAAGACGTTCAGGATGAGATTCTTACATTACCTGATGGTCTTTTGGCTCGATATTT
TAACTTAACTGACCGTATGGTGATTTACGGCGCAAACTTAGGAGAACCTCACACATTACCAATGAGTAAAGGACTATTTG
AGTTGCGCTTAAAATCTCAAGAAGGTATTGCTCGTGTTATGTATTGCACACTAGTCGGTAAACGCATTGTAATGCTCCAT
AGTTTTGTTAAGAAAACCCAAAAAACACCTAAGCAAGATCTAAACCTAGCACTTGACCGCATGAAAGAGGTGAAAAATGC
GAACACATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10565.30 Da
• Isoelectric Point: 10.1453

>AT97732 WP_001258993.1 NZ_CP027247:30411-30698 [Acinetobacter pittii]
MRTHEEMKALALSRSAVRTEYERIEREEMPLLDMVLTARRDAGLSQAQIAERMGTSVPAVSRLEKALITGKPSPSIATLQ
KYAAAIGKHVEVRFV

Nucleotide

• Length: 288 bp

>AT97732 NZ_CP027247:30411-30698 [Acinetobacter pittii]
ATGCGAACACATGAAGAAATGAAAGCACTTGCATTATCTCGTTCAGCAGTGCGTACCGAATACGAACGAATTGAACGTGA
AGAAATGCCCCTGCTCGATATGGTACTTACTGCACGTCGAGATGCAGGTCTATCCCAAGCGCAAATCGCGGAACGCATGG
GAACAAGTGTACCTGCCGTTTCACGGCTAGAAAAAGCATTGATCACAGGCAAACCATCACCATCTATTGCCACATTGCAA
AAATATGCTGCGGCAATTGGTAAACATGTTGAAGTGCGTTTTGTCTAA