Detailed information of TA system TA097731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 23546..24197
• Replicon: plasmid p1_100004
• Accession: NZ_CP027247
• Organism: Acinetobacter pittii strain WCHAP100004

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C6N19_RS00150
• Protein ID: WP_000269910.1
• Coordinates: 23546..23854 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6N19_RS00155
• Protein ID: WP_001140619.1
• Coordinates: 23895..24197 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N19_RS00115	19264..19698	+	435	WP_106405795.1	hypothetical protein	-
C6N19_RS00125	20886..21443	+	558	Protein_24	hypothetical protein	-
C6N19_RS00130	21700..21945	+	246	WP_000246757.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
C6N19_RS00135	21935..22228	+	294	WP_000221360.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C6N19_RS00140	22415..22531	+	117	Protein_27	transposase	-
C6N19_RS00150	23546..23854	+	309	WP_000269910.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6N19_RS00155	23895..24197	+	303	WP_001140619.1	XRE family transcriptional regulator	Antitoxin
C6N19_RS00160	24190..24399	+	210	WP_000069470.1	hypothetical protein	-
C6N19_RS00165	24509..24736	-	228	WP_000555567.1	hypothetical protein	-
C6N19_RS00170	24868..25671	-	804	WP_106405796.1	WYL domain-containing protein	-
C6N19_RS00175	25897..26225	+	329	Protein_33	DUF4236 domain-containing protein	-
C6N19_RS00180	26467..26979	-	513	WP_000464612.1	Fic family protein	-
C6N19_RS00185	26982..27167	-	186	WP_000755724.1	hypothetical protein	-
C6N19_RS00190	27334..28266	+	933	WP_000743263.1	IS5 family transposase	-
C6N19_RS00195	28397..28594	-	198	WP_025464206.1	hypothetical protein	-
C6N19_RS00200	28687..28905	-	219	WP_000994925.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..66765	66765
	-	flank	IS/Tn	-	-	27334..28266	932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-102)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11742.64 Da
• Isoelectric Point: 9.0002

>T97731 WP_000269910.1 NZ_CP027247:23546-23854 [Acinetobacter pittii]
MWTVITTDLFNEWLVQQDQSTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIAD

Nucleotide

• Length: 309 bp

>T97731 NZ_CP027247:23546-23854 [Acinetobacter pittii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGTACAGCAAGATCAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGACGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCGTTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11206.01 Da
• Isoelectric Point: 6.7282

>AT97731 WP_001140619.1 NZ_CP027247:23895-24197 [Acinetobacter pittii]
MAKTLQELLANRSPESQARIQQMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT97731 NZ_CP027247:23895-24197 [Acinetobacter pittii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAATCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCGGATGAATT
ATTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTGGAGATTTCTCAAAAAGAACTGGCTGCAACCCTTGGAATCA
AACAGCCTTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTCGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAATGTCTAA